Thiographene synthesized from fluorographene via xanthogenate with immobilized enzymes for environmental remediation. Issue 22 (23rd May 2019)

• Record Type: Journal Article
• Authors: Sturala, Jiri; Hermanová, Soňa; Artigues, Lucie; Sofer, Zdeněk; Pumera, Martin
• Is Part Of: Nanoscale. Volume 11:Issue 22(2019)
• Page Start: 10695
• Page End: 10701
• Publication Date: 2019-05-23
• Subjects: Nanoscience -- Periodicals; Nanotechnology -- Periodicals; 620.505
• DOI: 10.1039/c9nr02376c
• Languages: English
• ISSNs: 2040-3364

Abstract

Abstract : Thiographene with a high content of thiol groups and it was used as a platform for anchoring both hydrolytic and oxidoreductase-based enzymes to produce efficient biocatalytic systems with no leaking of immobilized enzymatic molecules during storage. Abstract : Graphene, graphene oxide and their related thiographene-, hydroxygraphene- or fluorographene-based materials have broad applications. We report on the thiol-functionalization of fluorographene via xanthogenate. Such thiographene contains 5.1 at% of sulphur in the form of thiol groups, which is the highest thiol content reported to date. Such tailored thiographene allows the immobilization of two types of enzymes. Here, we explore the functionalization of highly thiolated graphene with enzymes via physisorption or covalent linkage producing an important heterogeneous biocatalyst platform for wastewater treatment applications. Thiographene modified with a lipase from Mucor miehei can find utilization in lipid-rich wastewater treatment whereas the catalase-modified thiographene is intended for bioremediation applications. Upon increasing concentration of the thiol groups on graphene, protein loading of the catalase was increased by 16% and the ester bond cleavage activity of the thiographene-immobilized lipase was 129% that of the free lipase. We expect that such a highly active heterogeneous thiographene-based biocatalyst will find a use in water remediation applications.