Phytochemical Analysis and Anti-Inflammatory Activity of the Extracts of the African Medicinal Plant Ximenia caffra. (17th February 2015)

• Record Type: Journal Article
• Authors: Zhen, Jing; Guo, Yue; Villani, Tom; Carr, Steve; Brendler, Thomas; Mumbengegwi, Davis R.; Kong, Ah-Ng Tony; Simon, James E.; Wu, Qingli
• Other Names: Xie Mengxia Academic Editor.
• Is Part Of: Journal of analytical methods in chemistry. Volume 2015(2015)
• Publication Date: 2015-02-17
• Subjects: Chemistry, Analytic -- Periodicals; Chemistry, Analytic -- Technique -- Periodicals; 543.05
• DOI: 10.1155/2015/948262
• Languages: English
• ISSNs: 2090-8865

Abstract

Abstract : A method was developed for identification and quantification of polyphenols in the leaves of Ximenia caffra using HPLC/UV/MS. Based on analyzing the MS and UV data and in comparison to the authentic standards, a total of 10 polyphenols were identified and quantified, including gallic acid, catechin, quercetin, kaempferol, and their derivatives. The total content of these compounds was found to be approximately 19.45 mg/g in the leaf and the most abundant is quercetin-rutinoside (9.08 mg/g). The total phenolic content as measured by Folin-Ciocalteu assay was 261.87 ± 7.11 mg GAE/g and the total antioxidant capacity as measured in vitro was 1.46 ± 0.01 mmol Trolox/g. The antiproliferative effect of the leaf extract was measured by MTS assay with IC50 value of 239.0 ± 44.5 μ g/mL. Cell-based assays show that the leaf extract inhibits the mRNA expression of proinflammatory genes (IL-6, iNOS, and TNF- α ) by using RT-qPCR, implying its anti-inflammatory effects. It was further demonstrated that the underlying therapeutic mechanism involves the suppression of NF- κ B, a shared pathway between cell death and inflammation.