Hyaluronan-Based Three-Dimensional Microenvironment Potently Induces Cardiovascular Progenitor Cell Populations. (3rd September 2013)

• Record Type: Journal Article
• Authors: Gluck, Jessica M.; Chyu, Jennifer; Delman, Connor; Heydarkhan-Hagvall, Sepideh; MacLellan, W. Robb; Shemin, Richard J.
• Other Names: Haasper C. Academic Editor.; Ren Y.-J. Academic Editor.
• Is Part Of: ISRN tissue engineering. Volume 2013(2013)
• Publication Date: 2013-09-03
• Subjects: Tissue engineering -- Periodicals; 571.538
• DOI: 10.1155/2013/752620
• Languages: English
• ISSNs: 2314-4696

Abstract

Abstract : The relationship between stem cell niches in vivo and their surrounding microenvironment is still relatively unknown. Recent advances have indicated that extrinsic factors within the cardiovascular progenitor cell niche influence maintenance of a multipotent state as well as drive cell-fate decisions. We have previously shown the direct effects of extracellular matrix (ECM) proteins and have now investigated the effects of dimension on the induction of a cardiovascular progenitor cell (CPC) population. We have shown here that the three-dimensionality of a hyaluronan-based hydrogel greatly induces a CPC population, as marked by Flk-1. We have compared the effects of a 3D microenvironment to those of conventional 2D cell culture practices and have found that the 3D microenvironment potently induces a progenitor cell state.