Nanocomposites Based on PLLA and Multi Walled Carbon Nanotubes Support the Myogenic Differentiation of Murine Myoblast Cell Line. (1st July 2013)

• Record Type: Journal Article
• Authors: Montesano, Simona; Lizundia, Erlantz; D'Angelo, Francesco; Fortunati, Elena; Mattioli, Samantha; Morena, Francesco; Bicchi, Ilaria; Naro, Fabio; Sampaolesi, Maurilio; Sarasua, Josè Ramon; Kenny, Josè Maria; Orlacchio, Aldo; Armentano, Ilaria; Martino, Sabata
• Other Names: Haasper C. Academic Editor.; Mansbridge J. Academic Editor.; Ren Y.-J. Academic Editor.
• Is Part Of: ISRN tissue engineering. Volume 2013(2013)
• Publication Date: 2013-07-01
• Subjects: Tissue engineering -- Periodicals; 571.538
• DOI: 10.1155/2013/825912
• Languages: English
• ISSNs: 2314-4696

Abstract

Abstract : We explored the effect of poly(L-lactic acid) (PLLA) containing various percentages (0.1, 0.5, 1, and 3 wt.%) of multi walled carbon nanotubes (MWCNTs) on the myogenic differentiation of C2C12 murine myoblast progenitor cells. We showed that all PLLA/MWCNTs nanocomposite materials support the myotubes formation more efficiently than neat PLLA as indicated by the high expression of the most significant myogenic markers: MyoD, Myosin Heavy Chain, dimension of myofibres, and fusion myogenic index. Interestingly, we note that both MyoD and myogenic fusion index levels were in the order 0.1 MWCNTs = 0.5 MWCNTs > 1 MWCNTs > 3 MWCNTs > neat PLLA, suggesting that the amount of MWCNTs influenced the cell differentiation.