Phylogenetic Framework and Biosurfactant Gene Expression Analysis of Marine Bacillus spp. of Eastern Coastal Plain of Tamil Nadu. (12th February 2014)

• Record Type: Journal Article
• Authors: Swaathy, Sreethar; Kavitha, Varadharajan; Sahaya Pravin, Arockiasamy; Sekaran, Ganesan; Mandal, Asit Baran; Gnanamani, Arumugam
• Other Names: Mendes Rodrigo E. Academic Editor.
• Is Part Of: International journal of bacteriology. Volume 2014(2014)
• Publication Date: 2014-02-12
• Subjects: Bacteriology -- Periodicals; Bacterial Physiological Phenomena; Bacteriology; Periodical; Periodicals; Fulltext; Internet Resources; Periodicals; Electronic journals; 616.9201
• DOI: 10.1155/2014/860491
• Languages: English
• ISSNs: 2356-6957

Abstract

Abstract : The present study emphasizes the diversity assessment of marine Bacillus species with special reference to biosurfactant production, respective gene expression, and discrimination among Bacillus licheniformis and Bacillus subtilis . Among the 200 individual species of eastern coastal plain of Tamil Nadu screened, five biosurfactant producing potential bacterial species with entirely different morphology were selected. Biochemical and 16S rRNA gene sequence analysis suggested that all the said five species belong to Bacillus genera but differ in species levels. Biosurfactant of all the five species fluctuates in greater levels with respect to activity as well as to constituents but showed partial similarity to the commercially available surfactin. The expression of srf gene was realized in all of the five species. However, the sfp gene expression was observed only in three species. In conclusion, both B. licheniformis and B. subtilis demonstrate srf gene; nevertheless, sfp gene was expressed only by Bacillus subtilis .