Altered Norbin Expression in Patients with Epilepsy and a Rat Model. Issue 1 (December 2017)
- Record Type:
- Journal Article
- Title:
- Altered Norbin Expression in Patients with Epilepsy and a Rat Model. Issue 1 (December 2017)
- Main Title:
- Altered Norbin Expression in Patients with Epilepsy and a Rat Model
- Authors:
- Xu, Yali
Li, Zengyou
Yao, Li
Zhang, Xingping
Gan, Dan
Jiang, Manchun
Wang, Na
Chen, Guojun
Wang, Xuefeng - Abstract:
- Abstract Norbin is widely distributed in neuronal tissues, is a regulator of Ca2+ /calmodulin-dependent protein kinase II (CaMKII) phosphorylation. Norbin is also an important endogenous modulator of metabotropic glutamate receptor 5 (mGluR5) signaling, and nervous system-specific homozygous gene disruptions, result in epileptic seizures. In this study, we aimed to investigate norbin expression patterns in epilepsy and to elucidate the relationships between norbin and mGluR5 and p-CaMKII in epilepsy. Double-immunolabeling, immunohistochemistry and immunoblotting studies showed that norbin was downregulated in the temporal neocortex of patients with temporal lobe epilepsy (TLE) compared with control subjects. Moreover, in a rat model of lithium chloride-pilocarpine-induced epilepsy, norbin expression began to decrease at 6 h after the onset of status epilepticus and remained at a low level until 60 days. In addition, p-CaMKII expression was significantly increased in both patients with TLE and in animal model. Norbin and mGluR5 were found to be co-expressed in neurons of epileptic tissues. Finally, norbin over-expression facilitated by injections of adeno-associated viral vector into the rat hippocampus increased latency and survival in the lithium chloride-pilocarpine model. Thus, our results indicate norbin participates in the pathogenesis of epilepsy, perhaps by modulating mGluR5 signaling, regulating CaMKII phosphorylation, and may exert antiepileptic effects.
- Is Part Of:
- Scientific reports. Volume 7:Issue 1(2017)
- Journal:
- Scientific reports
- Issue:
- Volume 7:Issue 1(2017)
- Issue Display:
- Volume 7, Issue 1 (2017)
- Year:
- 2017
- Volume:
- 7
- Issue:
- 1
- Issue Sort Value:
- 2017-0007-0001-0000
- Page Start:
- 1
- Page End:
- 13
- Publication Date:
- 2017-12
- Subjects:
- Natural history -- Research -- Periodicals
Biology -- Research -- Periodicals
Physical sciences -- Research -- Periodicals
Earth sciences -- Research -- Periodicals
Environmental sciences -- Research -- Periodicals
502.85 - Journal URLs:
- http://www.nature.com/ ↗
http://www.nature.com/srep/index.html ↗ - DOI:
- 10.1038/s41598-017-13248-9 ↗
- Languages:
- English
- ISSNs:
- 2045-2322
- Deposit Type:
- Legaldeposit
- View Content:
- Available online (eLD content is only available in our Reading Rooms) ↗
- Physical Locations:
- British Library DSC - BLDSS-3PM
British Library HMNTS - ELD Digital store - Ingest File:
- 10821.xml