Role of the luxS gene in bacteriocin biosynthesis by Lactobacillus plantarum KLDS1.0391: A proteomic analysis. Issue 1 (December 2017)

• Record Type: Journal Article
• Authors: Jia, Fang-Fang; Pang, Xue-Hui; Zhu, De-Quan; Zhu, Zong-Tao; Sun, Si-Rui; Meng, Xiang-Chen
• Is Part Of: Scientific reports. Volume 7:Issue 1(2017)
• Page Start: 1
• Page End: 14
• Publication Date: 2017-12
• Subjects: Natural history -- Research -- Periodicals; Biology -- Research -- Periodicals; Physical sciences -- Research -- Periodicals; Earth sciences -- Research -- Periodicals; Environmental sciences -- Research -- Periodicals; 502.85
• DOI: 10.1038/s41598-017-13231-4
• Languages: English
• ISSNs: 2045-2322

Abstract

Abstract Certain probiotic species of lactic acid bacteria, especiallyLactobacillus plantarum, regulate bacteriocin synthesis through quorum sensing (QS) systems. In this study, we aimed to investigate theluxS -mediated molecular mechanisms of QS during bacteriocin synthesis byL. plantarum KLDS1.0391. In the absence ofluxS, the 'spot-on-the-lawn' method showed that the bacteriocin production byL. plantarum KLDS1.0391 significantly decreased upon co-cultivation withL. helveticus KLDS1.9207 (P < 0.01) but did not change significantly when mono-cultivated. Furthermore, liquid chromatography-electrospray ionization tandem mass spectrometry analysis showed that, as a response toluxS deletion, L. plantarum KLDS1.0391 altered the expression level of proteins involved in carbohydrate metabolism, amino acid metabolism, fatty acid synthesis and metabolism, and the two-component regulatory system. In particular, the sensor histidine kinase AgrC (from the two-component system, LytTR family) was expressed differently between theluxS mutant and the wild-type strain during co-cultivation, whereas no significant differences in proteins related to bacteriocin biosynthesis were found upon mono-cultivation. In summary, we found that the production of bacteriocin was regulated by carbohydrate metabolism, amino acid metabolism, fatty acid synthesis and metabolism, and the two-component regulatory system. Furthermore, our results demonstrate the role ofluxS -mediated molecular mechanisms in bacteriocin production.