Covalent Attachment of Carbohydrate Derivatives to an Evanescent Wave Fiber Bragg Grating Biosensor. (8th November 2009)

• Record Type: Journal Article
• Authors: Stanford, Christopher J.; Ryu, Geunmin; Dagenais, Mario; Hurley, Matthew T.; Gaskell, Karen J.; DeShong, Philip
• Other Names: Riziotis Christos Academic Editor.
• Is Part Of: Journal of sensors. Volume 2009(2009)
• Publication Date: 2009-11-08
• Subjects: Detectors -- Periodicals; 681.205
• DOI: 10.1155/2009/982658
• Languages: English
• ISSNs: 1687-725X

Abstract

Abstract : A carbohydrate-based biosensor was prepared by functionalization of the surface of an etched fiber Bragg grating with a glucopyranosyl-siloxane conjugate. Functionalization of the surface with the conjugate resulted in a Bragg grating shift of 24 pm. This shift in the refractive index is consistent with a theoretical shift calculated assuming monolayer coverage of the glucose conjugate on the sensor. The resulting functionalized fiber was shown to interact selectively with concanavalin A (Con A), a glucose binding protein (lectin). Exposure of the glucose-functionalized fiber to peanut agglutinin, a galactosebinding lectin, did not result in a change of the refractive index corresponding to a binding event.