Bio-artificial pleura using an autologous dermal fibroblast sheet. (December 2017)

• Record Type: Journal Article
• Authors: Kanzaki, Masato; Takagi, Ryo; Washio, Kaoru; Kokubo, Mami; Yamato, Masayuki
• Is Part Of: Npj regenerative medicine. Volume 2(2017)
• Page Start: 1
• Page End: 2
• Publication Date: 2017-12
• Subjects: Regenerative medicine -- Periodicals; 611.018
• DOI: 10.1038/s41536-017-0031-2
• Languages: English
• ISSNs: 2057-3995

Abstract

Abstract Air leaks (ALs) are observed after pulmonary resections, and without proper treatment, can produce severe complications. AL prevention is a critical objective for managing patients after pulmonary resection. This study applied autologous dermal fibroblast sheets (DFS) to close ALs. For sealing ALs in a 44-year-old male human patient with multiple bullae, a 5 × 15-mm section of skin was surgically excised. From this skin specimen, primary dermal fibroblasts were isolated and cultured for 4 weeks to produce DFSs that were harvested after a 10-day culture. ALs were completely sealed using surgical placement of these autologous DFSs. DFS were found to be a durable long-term AL sealant, exhibiting requisite flexibility, elasticity, durability, biocompatibility, and usability, resulting reliable AL closure. DFS should prove to be an extremely useful tissue-engineered pleura substitute.