High resolution UPLC-MS/MS profiling of polyphenolics in the methanol extract of Syzygium samarangense leaves and its hepatoprotective activity in rats with CCl4-induced hepatic damage. (March 2018)

• Record Type: Journal Article
• Authors: Sobeh, Mansour; Youssef, Fadia S.; Esmat, Ahmed; Petruk, Ganna; El-Khatib, Ahmed H.; Monti, Daria Maria; Ashour, Mohamed L.; Wink, Michael
• Is Part Of: Food and chemical toxicology. Volume 113(2018)
• Page Start: 145
• Page End: 153
• Publication Date: 2018-03
• Subjects: Antioxidant -- Carbon tetrachloride toxicity -- HaCaT cells -- Hepatoprotective activity -- HR-UPLC-MS/MS -- Syzygium samarangense; ABTS+ 2, 2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid -- ALT alanine transaminase -- ANOVA one way analysis of variance -- AST aspartate transaminase -- CCl4 carbon tetrachloride -- DCFDA 2′, 7′-dichlorodihydrofluorescein diacetate -- DPPH 2, 2-diphenyl-1-picrylhydrazyl radical -- DTNB 5, 5′-dithiobis-2-nitrobenzoic acid -- ESI electrospray ionization -- FRAP Ferric reducing antioxidant power -- GAE gallic acid equivalent -- GSH reduced glutathione -- HaCaT cells normal human keratinocytes -- HR-UPLC-MS/MS high resolution ultra-high performance liquid chromatography mass-mass spectrometry -- MDA malondialdehyde -- MTT 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide -- ROS reactive oxygen species -- SOD superoxide dismutase -- TB total bilirubin -- TC total cholesterol -- TEAC Trolox equivalent antioxidant capacity -- TG total glycerides -- UVA near ultra violet radiation (315–400 nm); Toxicology -- Periodicals; Food poisoning -- Periodicals; Food Poisoning -- Periodicals; Toxicology -- Periodicals; Toxicologie -- Périodiques; Intoxications alimentaires -- Périodiques; Food poisoning; Toxicology; Periodicals; Electronic journals; 615.9
• DOI: 10.1016/j.fct.2018.01.031
• Languages: English
• ISSNs: 0278-6915

Abstract

Abstract: Oxidative stress plays a crucial role in the development of several liver diseases. Many natural polyphenols can attenuate oxidative stress and liver injury. In this study, a phytochemical profiling of a methanol extract from leaves of Syzygium samarangense revealed 92 compounds belonging to flavonoids, phenolic acids, condensed tannins, and ellagitannins. The S. samarangense extract exhibited a noticeable antioxidant activity with an EC50 of 5.80 μg/mL measured by DPPH scavenging capacity assay, 2632 Trolox equivalents, 10 mM Fe 2+ equivalents/mg of samples by TEAC and FRAP assays, respectively. The total phenolic content was 419 mg gallic acid equivalent GAE/g extract. In a cell-based model (HaCaT cells), the extract completely inhibited ROS production induced by UVA, and prevented GSH-depletion and p38 phosphorylation. In addition, the extract exhibited a substantial antioxidant and hepatoprotective activities in CCl4 -treated rats, with an increase in GSH (reduced glutathione) and SOD (superoxide dismutase) activities by 84.75 and 26.27%, respectively, and a decrease of 19.08, 63.05, 52.21, 37.00, 13.26, and 15.15% in MDA, ALT, AST, TB (total bilirubin), TC (total cholesterol), and TG (total glycerides), respectively. These results were confirmed by histopathological analyses. We believe that Syzygium samarangense is a good candidate for further evaluation as an antioxidant and liver protecting drug. Highlights: 92 polyphenolics were identified by UPLC-MS/MS from Syzygium samarangense leaves. Phenols included flavonol glycosides, proanthocyanidins, and ellagitannins. The extract showed potent antioxidant activity in vitro and on human keratinocytes. The extract showed a hepatoprotective potential similarly to silymarin.