Anti-inflammatory effects of soyasapogenol I-αa via downregulation of the MAPK signaling pathway in LPS-induced RAW 264.7 macrophages. (March 2018)

• Record Type: Journal Article
• Authors: Yang, Seung Hwan; Le, Bao; Androutsopoulos, Vasilis P.; Tsukamoto, Chigen; Shin, Tae-Sun; Tsatsakis, Aristides M.; Chung, Gyuhwa
• Is Part Of: Food and chemical toxicology. Volume 113(2018)
• Page Start: 211
• Page End: 217
• Publication Date: 2018-03
• Subjects: Cytokines -- Glycine soja -- Inflammatory -- Macrophage -- Soyasaponin; COX-2 cyclooxygenase 2 -- DDMP 2, 3-dihydro-2, 5-dihydroxy-6-methyl-4-pyrone -- DMEM Dulbecco's modified Eagle's medium -- ERK extracellular signal-regulated kinases -- FBS fetal bovine serum -- iNOS inducible nitric oxide synthase -- JNK c-Jun N-terminal kinase -- LPS lipopolysaccharides -- MAPKs mitogen-activated protein kinase -- MTT 3-[4, 5-dimethylthiazol-2-yl]-2, 5 diphenyl tetrazolium bromide -- PBS phosphate-buffered saline -- PDA photodiode-array detector; Toxicology -- Periodicals; Food poisoning -- Periodicals; Food Poisoning -- Periodicals; Toxicology -- Periodicals; Toxicologie -- Périodiques; Intoxications alimentaires -- Périodiques; Food poisoning; Toxicology; Periodicals; Electronic journals; 615.9
• DOI: 10.1016/j.fct.2018.01.050
• Languages: English
• ISSNs: 0278-6915

Abstract

Abstract: The crude extract of soyasaponins was reported to possess anti-inflammatory activity. We determined the new purity group I saponin, I-αa and I-γa that was isolated from wild soybean ( Glycine soja ) in terms of its efficacy in protecting RAW 264.7 macrophages from lipopolysaccharide (LPS)-stimuli. Cells were treated with soyasaponin I-αa/I-γa (30–300 μΜ) and LPS (0.1 μg/mL) for 24 h. Soyasaponin I-αa inhibited nitric oxide (NO) production at 100 μg/mL, while soyasaponin I-γa demonstrated this effect at a higher concentration (200 μg/mL). The expression levels of iNOS and COX-2 enzymes were downregulated by both soyasaponins. Soyasaponin I-αa exerted its effect via the TNF-α and IL-1β cytokines. However, soyasaponin I-γa only inhibited the expression of TNF-α. The inflammatory effect of group I soyasaponin was mainly mediated via the phosphorylation of the p38 and JNK proteins. Collectively, these results suggested the potential anti-inflammatory effects of soyasaponins. Graphical abstract: Highlights: Anti-inflammatory effects of a group I saponins from Glycine soja are reported. Soyasaponin I-αa inhibited the TNF-α and IL-1β cytokine induced NO generation in RAW 264.7 macrophages. Soyasaponin I-γa only inhibited the expression of TNF-α. Group I soyasaponin stimulated anti-inflammatory effects via mechanisms related to the p38 and JNK signaling pathways.