Rapid one-pot fabrication of magnetic calcium phosphate nanoparticles immobilizing DNA and iron oxide nanocrystals using injection solutions for magnetofection and magnetic targeting. (December 2017)

• Record Type: Journal Article
• Authors: Shubhra, Quazi T.H.; Oyane, Ayako; Nakamura, Maki; Puentes, Sandra; Marushima, Aiki; Tsurushima, Hideo
• Is Part Of: Materials today chemistry. Volume 6(2017)
• Page Start: 51
• Page End: 61
• Publication Date: 2017-12
• Subjects: Magnetofection -- Infusion fluid -- Gene delivery -- Calcium phosphate -- Nanoparticles -- Supersaturated solution; Chemistry -- Periodicals; Materials -- Research -- Periodicals; Materials science -- Periodicals; Chemistry; Materials -- Research; Electronic journals; Periodicals; 660.282
• DOI: 10.1016/j.mtchem.2017.10.001
• Languages: English
• ISSNs: 2468-5194

Abstract

Abstract: This is the first report on rapid (5–30 min) one-pot fabrication of magnetic calcium phosphate (CaP) nanoparticles (NPs) co-immobilizing DNA and iron oxide (IO) nanocrystals with high immobilization efficiencies (DNA (∼90%) and IO (∼70%)). NPs were fabricated via coprecipitation under clean bench in supersaturated CaP solutions using DNA, IO-based MRI contrast agent, and infusion quality source solutions (even the water used was of injectable quality) that ensured high safety level of the fabrication process and product. Prepared DNA-IO-CaP NPs exhibited strong magnetic property allowing their noncontact manipulation by external magnet. These NPs were smaller than 500 nm, had relatively large negative zeta potential showing stable dispersion without any additional surfactant, and exhibited no significant cytotoxicity under the tested transfection conditions. The IO content of these NPs was significantly varied and by adjusting the initial IO concentration, DNA-IO-CaP NPs with gene delivery capability to CHO-K1 cells as high as that of the IO-free DNA-CaP NPs without external magnetic field were prepared. Fabricated DNA-IO-CaP NPs showed significantly improved gene delivery capability under external magnetic field compared to the IO-free DNA-CaP NPs. Thus, the present coprecipitation process can be considered as a novel technique to fabricate multifunctional CaP-based NPs to achieve magnetofection and targeted delivery. Graphical abstract: Highlights: We prepared calcium phosphate nanoparticles containing DNA & γ-Fe2 O3 nanocrystals. Nanoparticles were prepared by a rapid one-pot process using injectable fluids. Nanoparticles showed magnetic property, stable dispersion, and no cytotoxicity. Fe content of nanoparticles was varied which affected gene delivery capability. Nanoparticles showed improved delivery capability under the magnetic field.