Comparative proteomic analysis of hepatic effects induced by nanosilver, silver ions and nanoparticle coating in rats. (March 2018)

• Record Type: Journal Article
• Authors: Juling, Sabine; Böhmert, Linda; Lichtenstein, Dajana; Oberemm, Axel; Creutzenberg, Otto; Thünemann, Andreas F.; Braeuning, Albert; Lampen, Alfonso
• Is Part Of: Food and chemical toxicology. Volume 113(2018)
• Page Start: 255
• Page End: 266
• Publication Date: 2018-03
• Subjects: Silver nanoparticles NM-300 -- Silver acetate -- Coating -- 2-D gel MALDI-TOF MS/MS -- In vivo -- Proteomics; Toxicology -- Periodicals; Food poisoning -- Periodicals; Food Poisoning -- Periodicals; Toxicology -- Periodicals; Toxicologie -- Périodiques; Intoxications alimentaires -- Périodiques; Food poisoning; Toxicology; Periodicals; Electronic journals; 615.9
• DOI: 10.1016/j.fct.2018.01.056
• Languages: English
• ISSNs: 0278-6915

Abstract

Abstract: The presence of nano-scaled particles in food and food-related products has drawn attention to the oral uptake of nanoparticles and their interactions with biological systems. In the present study, we used a toxicoproteomics approach to allow for the untargeted experimental identification and comparative analysis of cellular responses in rat liver after repeated-dose treatment with silver nanoparticles, ions, and the coating matrix used for particle stabilization. The proteomic analysis revealed treatment-related effects caused by exposure to silver in particulate and ionic form. Both silver species induced similar patterns of signaling and metabolic alterations. Silver-induced cellular alterations comprised, amongst others, proteins involved in metal homeostasis, oxidative stress response, and energy metabolism. However, we discovered that secondary nano-scaled structures were formed from ionic silver. Furthermore, also the coating matrix alone gave rise to the formation of nano-scaled particles. The present data confirm, complement, and extend previous knowledge on silver toxicity in rodent liver by providing a comprehensive proteomic data set. The observation of secondary particle formation from non-particle controls underlines the difficulties in separating particle-, ion-, and matrix coating-related effects in biological systems. Awareness of this issue will support proper evaluation of nanotoxicology-related data in the future. Highlights: Proteomic analysis of nanosilver effects in vivo. Similar effects exerted by particulate and ionic silver. Ionic silver forms secondary nano-scaled structures. Silver influences oxidative stress response, energy metabolism and metal homeostasis of the cell.