Baicalein improves insulin resistance via regulating SOCS3 and enhances the effect of acarbose on diabetes prevention. (October 2017)

• Record Type: Journal Article
• Authors: Sun, Wenlong; Sun, Jin; Zhang, Bowei; Xing, Yan; Yu, Xiaoxia; Li, Xia; Xiu, Zhilong; Dong, Yuesheng
• Is Part Of: Journal of functional foods. Volume 37(2017)
• Page Start: 339
• Page End: 353
• Publication Date: 2017-10
• Subjects: 2h-PG 2-hour postprandial glucose -- ALT alanine transaminase -- AUC area under the curve -- AST aspartate transaminase -- JNK C-Jun N-terminal kinase -- DMEM Dulbecco's modified Eagle's medium -- FBG fasting blood glucose -- GPx glutathione peroxidase -- HDLC high density lipoprotein cholesterol -- IFG impaired fasting glycaemia -- IGT impaired glucose tolerance -- Ikkβ inhibitor of nuclear factor kappa-B kinase β -- LDLC low density lipoprotein cholesterol -- NGT normal glucose tolerance -- OGTT oral glucose tolerance test -- OISE Oroxylum indicum seed extract -- PPARγ peroxisome proliferator-activated receptor γ -- PMSF phenylmethanesulfonyl fluoride -- PBS phosphate-buffered saline -- PVDF polyvinylidene fluoride -- PTP1b protein tyrosine phosphatase 1b -- SDS-PAGE SDS–polyacrylamide gel electrophoresis -- CMC-Na sodium carboxymethylcellulose -- SOD superoxide dismutase -- SOCS3 suppressor of cytokine signaling 3 -- RRRD the relative risk reduction of progression from prediabetes to diabetes -- T-AOC total antioxidative capacity -- TC total cholesterol -- TG triglyceride -- TBST tris buffered saline with Tween-20 -- T2DM type 2 diabetes mellitus; Baicalein -- Dietary supplement -- Acarbose -- SOCS3 -- Insulin sensitizer -- Diabetes prevention; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2017.08.005
• Languages: English
• ISSNs: 1756-4646

Abstract

Highlights: Baicalein from S. baicalensis and O. indicum is used as food supplements in Asia. Baicalein improves insulin resistance by regulating SOCS3 in vitro and in vivo . Moderate baicalein consumption reduces the side-effects of acarbose. Baicalein improves the effect of acarbose on diabetes prevention. Abstract: Baicalein, a major component in Scutellaria baicalensis and Oroxylum indicum, is used as dietary supplement in Asia. In this study, we characterized the insulin-sensitizing activity of baicalein and found that baicalein improved insulin resistance by reducing the expression of SOCS3, increasing the phosphorylation of IRS1 and Akt1, reducing the phosphorylation of GSK3β and increasing hepatic glycogen level. We further evaluated the preventive effect of baicalein combined with acarbose in mice, aiming to enhance the therapeutic effect and reduce the side-effects of acarbose. The combination reduced the relative risk of progression from prediabetes to diabetes by 83.3% and the dose of acarbose by 80%. The underlying mechanisms include reducing postprandial hyperglycemia, improving insulin resistance, improving lipid metabolism, and reducing oxidative stress. Our results suggest that the consumption of 195–780 mg/d baicalein might improve the efficacy of acarbose on diabetes prevention and reduce the side-effects of acarbose in long-term acarbose users.