Bioassay-guided isolation of glycoprotein SPG-56 from sweet potato Zhongshu-1 and its anti-colon cancer activity in vitro and in vivo. (August 2017)

• Record Type: Journal Article
• Authors: Wang, Meimei; Ma, Hang; Tian, Cheng; Liu, Shanshan; Ye, Xiaoli; Zhou, Deqi; Li, Yanlin; Hui, Nie; Li, Xuegang
• Is Part Of: Journal of functional foods. Volume 35(2017)
• Page Start: 315
• Page End: 324
• Publication Date: 2017-08
• Subjects: Sweet potato -- Glycoprotein -- Bio-guided isolation -- Colon cancer -- Apoptosis; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2017.05.049
• Languages: English
• ISSNs: 1756-4646

Abstract

Highlights: A new glycoprotein SPG-56 was firstly separated from a new sweet potato variety Zhongshu-1. Glycoprotein SPG-56 was separated by tracking of bioactivity. Electrophoresis was used for the quantitative preparation of SPG-56. Both vitro and vivo anti-colon cancer activities of SPG-56 were evaluated. Abstract: This study is aimed at separating and identifying a new glycoprotein from sweet potato (SPG) and detecting its anti-colon cancer bioactivity. The SPG-56 was obtained by water extraction, isoelectric precipitation, chromatography and electrophoresis. The compositions of sugar and protein in SPG-56 were identified with high performance liquid chromatography and amino acid analyzer, and the antitumor activities were explored using BALB/c nude mice and human colon cancer cells (HCT-116). Results showed that the molecular weight of SPG-56 is 56 KD. Relative sugar and protein contents of SPG-56 are 2.9% and 97.1%, comprising more than 6 types of sugars and 15 kinds of amino acids. Animal experiments showed that orally administrated SPG-56 could obviously suppress the tumor growth. Results from flow cytometry, western blotting and immunohistochemistry indicated that SPG-56 inhibits cancer by inducing apoptosis. These results suggest that SPG-56 would potentially delay the development of colon cancer.