Dietary compound proanthocyanidins from Chinese bayberry (Myrica rubra Sieb. et Zucc.) leaves inhibit angiogenesis and regulate cell cycle of cisplatin-resistant ovarian cancer cells via targeting Akt pathway. (January 2018)

• Record Type: Journal Article
• Authors: Zhang, Yu; Chen, Shiguo; Wei, Chaoyang; Rankin, Gary O.; Rojanasakul, Yon; Ren, Ning; Ye, Xingqian; Chen, Yi Charlie
• Is Part Of: Journal of functional foods. Volume 40(2018)
• Page Start: 573
• Page End: 581
• Publication Date: 2018-01
• Subjects: Proanthocyanidins -- Chinese bayberry leaves -- Ovarian cancer -- Angiogenesis -- Cell cycle -- Akt; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2017.11.045
• Languages: English
• ISSNs: 1756-4646

Abstract

Highlights: BLPs reduced cell viability and levels of VEGF, HIF-1α and ROS in A2780/CP70 cells. BLPs reduced tube formation in HUVEC cells and inhibited wound healing ability. BLPs targeted at Akt/mTOR/p70S6K/4E-BP1 pathway to inhibit angiogenesis. BLPs targeted at Akt, c-Myc, cyclin D1 and CDK4 to induce G1 cell cycle arrest. BLPs could be a valuable source of functional foods for cancer treatment. Abstract: Ovarian cancer is the leading cause of death from gynecological malignancy and natural products have drawn great attention for cancer treatment. Chinese bayberry leaves proanthocyanidin (BLPs) with epigallocatechin-3-O-gallate (EGCG) as its terminal and major extension units is unusual in the plant kingdom. In the present study, BLPs showed strong growth inhibitory effects on cisplatin-resistant A2780/CP70 cells by inhibiting angiogenesis and inducing G1 cell cycle arrest. BLPs reduced the tube formation in HUVECs and attenuated the wound healing ability in A2780/CP70 cells. BLPs further reduced the level of ROS and targeted Akt/mTOR/p70S6K/4E-BP-1 pathway to reduce the expression of HIF-1α and VEGF, and thus inhibited angiogenesis. Furthermore, BLPs induced G1 cell cycle arrest by reducing the expressions of c-Myc, cyclin D1 and CDK4, which was also in accordance with the flow cytometry analysis. Overall, these results indicated that BLPs could be a valuable resource of natural compounds for cancer treatment.