Γ-Mangostin, a xanthone from mangosteen, attenuates oxidative injury in liver via NRF2 and SIRT1 induction. (January 2018)

• Record Type: Journal Article
• Authors: Wang, Anqi; Li, Dan; Wang, Shengpeng; Zhou, Fayang; Li, Peng; Wang, Yitao; Lin, Ligen
• Is Part Of: Journal of functional foods. Volume 40(2018)
• Page Start: 544
• Page End: 553
• Publication Date: 2018-01
• Subjects: ALT alanine aminotransferase -- ARE antioxidant responsive element -- AST aspartate transaminase -- ATRA all--retinoid acid -- CCl4 carbon tetrachloride -- DCFH-DA 2′, 7′-dichlorofluorescin diacetate -- γ-man γ-mangostin -- GSH glutathione -- HO-1 heme oxygenase-1 -- JC-1 5, 5′, 6, 6′-tetrachloro-1, 1′, 3, 3′-tetraethyl-imidacarbocyanine iodide -- KEAP1 Kelch-like ECH-associated protein 1 -- MTT 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyl-2H-tetrazolium bromide -- NRF2 nuclear factor erythroid 2-related factor 2 -- PEG 400 polyethylene glycol 400 -- PFA paraformaldehyde -- PGC-1α peroxisome proliferators-activated receptor-γ coactivator-1α -- PPARγ peroxisome proliferator-activated receptor γ -- ROS reactive oxygen species -- SIRT1 silent mating type information regulation 2 homolog 1 -- SOD superoxide dismutase -- t-BHP tert-butyl hydroperoxide; γ-mangostin -- Mangosteen -- Liver oxidative injury -- NRF2 -- SIRT1 -- PGC-1α; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2017.11.047
• Languages: English
• ISSNs: 1756-4646

Abstract

Highlights: A xanthone, γ-mangostin, was obtained from the pericarps of Garcinia mangostana L. γ-Mangostin protects hepatocytes from oxidative injury in vitro and in vivo . γ-Mangostin causes nuclear translocation of NRF2 to enhance antioxidant capacity. γ-Mangostin increases the expression and deacetylation activity of SIRT1. γ-Mangostin activates PGC-1α to enhance mitochondrial function. Abstract: γ-Mangostin (γ-man), an active compound from Garcinia mangostana L., has been discovered as a hepatoprotective agent against oxidative injury. However, the underlying mechanisms remained unclear. The current study showed that γ-man stimulated the nuclear translocation of nuclear factor erythroid 2-related factor 2 (NRF2) to enhance antioxidant capacity under oxidative stress, which was partially reversed by treatment of the NRF2 inhibitor, all-trans-retinoic acid. Moreover, γ-man increased the expression and activity of SIRT1 (silent mating type information regulation 2 homolog 1), which facilitated the deacetylation of peroxisome proliferator-activated receptor γ coactivator 1α to improve the mitochondrial function in L02 cells. The protective effect of γ-man was partially blocked by treatment of the SIRT1 inhibitor tenovin-1 or SIRT1 knockdown. In vivo studies showed γ-man protected mice from carbon tetrachloride-induced acute liver injury, through up-regulation of NRF2 and SIRT1. Thus, γ-man might be a candidate to protect liver from acute oxidative injury.