Fabricating PLGA microparticles with high loads of the small molecule antioxidant N‐acetylcysteine that rescue oligodendrocyte progenitor cells from oxidative stress. Issue 1 (6th October 2017)

• Record Type: Journal Article
• Authors: Murphy, Nicholas P.; Lampe, Kyle J.
• Is Part Of: Biotechnology and bioengineering. Volume 115:Issue 1(2018)
• Page Start: 246
• Page End: 256
• Publication Date: 2017-10-06
• Subjects: controlled release -- N‐acetylcysteine -- oligodendrocyte progenitor cells -- oxidative stress -- PLGA -- reactive oxygen species; Biotechnology -- Periodicals; Bioengineering -- Periodicals; 660.6
• DOI: 10.1002/bit.26443
• Languages: English
• ISSNs: 0006-3592

Abstract

Abstract: Reactive oxygen species (ROS), encompassing all oxygen radical or non‐radical oxidizing agents, play key roles in disease progression. Controlled delivery of antioxidants is therapeutically relevant in such oxidant‐stressed environments. Encapsulating small hydrophilic molecules into hydrophobic polymer microparticles via traditional emulsion methods has long been a challenge due to rapid mass transport of small molecules out of particle pores. We have developed a simple alteration to the existing water‐in‐oil‐in‐water (W/O/W) drug encapsulation method that dramatically improves loading efficiency: doping external water phases with drug to mitigate drug diffusion out of the particle during fabrication. PLGA microparticles with diameters ranging from 0.6 to 0.9 micrometers were fabricated, encapsulating high loads of 0.6–0.9 µm diameter PLGA microparticles were fabricated, encapsulating high loads of the antioxidant N‐acetylcysteine (NAC), and released active, ROS‐scavenging NAC for up to 5 weeks. Encapsulation efficiencies, normalized to the theoretical load of traditional encapsulation without doping, ranged from 96% to 400%, indicating that NAC‐loaded external water phases not only prevented drug loss due to diffusion, but also doped the particles with additional drug. Antioxidant‐doped particles positively affected the metabolism of oligodendrocyte progenitor cells (OPCs) under H2 O2 ‐mediated oxidative stress when administered both before (protection) or after (rescue) injury. Antioxidant doped particles improved outcomes of OPCs experiencing multiple doses of H2 O2 by increasing the intracellular glutathione content and preserving cellular viability relative to the injury control. Furthermore, antioxidant‐doped particles preserve cell number, number of process extensions, cytoskeletal morphology, and nuclear size of H2 O2 ‐stressed OPCs relative to the injury control. These NAC‐doped particles have the potential to provide temporally‐controlled antioxidant therapy in neurodegenerative disorders such as multiple sclerosis (MS) that are characterized by continuous oxidative stress. Abstract : Modifying the traditional W/O/W method to incorporate small‐molecule drug in external water phases yields highly loaded PLGA microparticles encapsulating N‐acetyl cysteine (NAC) that release NAC and scavenge free radicals for at least 5 weeks. These antioxidant doped microparticles protect and rescue OPCs from exposure to oxidative stress: increasing glutathione content, metabolic activity, cell number, and process extensions above levels observed in injured cells.