Transcription factors in eukaryotic cells can functionally regulate gene expression by acting in oligomeric assemblies formed from an intrinsically disordered protein phase transition enabled by molecular crowding. Issue 5 (20th October 2018)

• Record Type: Journal Article
• Authors: Leake, Mark C.
• Is Part Of: Transcription. Volume 9:Issue 5(2018)
• Page Start: 298
• Page End: 306
• Publication Date: 2018-10-20
• Subjects: Gene expression -- transcription factors -- single-molecule -- super-resolution -- cell signaling -- intrinsically disordered protein -- phase transition -- molecular crowding -- fluorescent protein; Genetic transcription -- Periodicals; 572.8
• DOI: 10.1080/21541264.2018.1475806
• Languages: English
• ISSNs: 2154-1264

Abstract

ABSTRACT: High-speed single-molecule fluorescence microscopy in vivo shows that transcription factors in eukaryotes can act in oligomeric clusters mediated by molecular crowding and intrinsically disordered protein. This finding impacts on the longstanding puzzle of how transcription factors find their gene targets so efficiently in the complex, heterogeneous environment of the cell. Abbreviations CDF - cumulative distribution function; FRAP - fluorescence recovery after photobleaching; GFP - Green fluorescent protein; STORM - stochastic optical reconstruction microscopy; TF - Transcription factor; YFP - Yellow fluorescent protein