Stability engineering of the human antibody repertoire. Issue 2 (28th November 2013)

• Record Type: Journal Article
• Authors: Rouet, Romain; Lowe, David; Christ, Daniel
• Is Part Of: FEBS letters. Volume 588:Issue 2(2014)
• Page Start: 269
• Page End: 277
• Publication Date: 2013-11-28
• Subjects: Protein engineering -- Antibody therapeutics -- Protein aggregation -- Stability; Biochemistry -- Periodicals; Biophysics -- Periodicals; Molecular biology -- Periodicals; Biochimie -- Périodiques; Biochemistry; Biophysics; Molecular biology; Periodicals; 572.05
• DOI: 10.1016/j.febslet.2013.11.029
• Languages: English
• ISSNs: 0014-5793

Abstract

Abstract : Human monoclonal antibodies often display limited thermodynamic and colloidal stabilities. This behavior hinders their production, and places limitations on the development of novel formulation conditions and therapeutic applications. Antibodies are highly diverse molecules, with much of the sequence variation observed within variable domain families and, in particular, their complementarity determining regions. This has complicated the development of comprehensive strategies for the stability engineering of the human antibody repertoire. Here we provide an overview of the field, and discuss recent advances in the development of robust and aggregation resistant antibody therapeutics.