A flash in the pan: Dissecting dynamic amyloid intermediates using fluorescence. Issue 8 (1st March 2013)

• Record Type: Journal Article
• Authors: Nath, Abhinav; Rhoades, Elizabeth
• Is Part Of: FEBS letters. Volume 587:Issue 8(2013)
• Page Start: 1096
• Page End: 1105
• Publication Date: 2013-03-01
• Subjects: AD -- Alzheimer's disease -- ANS -- 1-anilinonaphthalene-8-sulfonic acid -- AS -- α-synuclein -- CMC -- critical micellar concentration -- CTE -- chronic traumatic encephalopathy -- EPR -- electron paramagnetic -- ETeff -- energy transfer efficiency -- FRET -- Förster resonance energy transfer -- IAPP -- islet amyloid polypeptide -- IDP -- intrinsically disordered protein -- MTBR -- microtubule-binding region -- NFT -- neurofibrillary tangle -- NMR -- nuclear magnetic resonance -- PD -- Parkinson's disease -- PheCN -- p-cyanophenylalanine -- PRR -- proline-rich region -- SDS -- sodium dodecyl sulfate -- T2DM -- type II diabetes mellitus -- TCCD -- two-color coincidence detection -- ThT -- thioflavin-T -- Amyloid -- Intrinsically disordered protein -- Misfolding -- Aggregation -- FRET; Biochemistry -- Periodicals; Biophysics -- Periodicals; Molecular biology -- Periodicals; Biochimie -- Périodiques; Biochemistry; Biophysics; Molecular biology; Periodicals; 572.05
• DOI: 10.1016/j.febslet.2013.02.044
• Languages: English
• ISSNs: 0014-5793

Abstract

Abstract : Several widespread and severe degenerative diseases are characterized by the deposition of amyloid protein aggregates in affected tissues. While there is great interest in the complete description of the aggregation pathway of the proteins involved, a molecular level understanding is hindered by the complexity of the self‐assembly process. In particular, the early stages of aggregation, where dynamic, heterogeneous and often toxic intermediates are populated, are resistant to high‐resolution structural characterization. Fluorescence spectroscopy is a powerful and versatile tool for such analysis. In this review, we survey its application to provide residue‐specific information about amyloid intermediate states for three selected proteins: IAPP, α‐synuclein, and tau.