Monitoring of the ADP/ATP Ratio by Induced Circularly Polarised Europium Luminescence. (14th May 2018)

• Record Type: Journal Article
• Authors: Shuvaev, Sergey; Fox, Mark A.; Parker, David
• Is Part Of: Angewandte Chemie. Volume 130:Number 25(2018)
• Page Start: 7610
• Page End: 7614
• Publication Date: 2018-05-14
• Subjects: Lanthanoide -- Lumineszenz -- Lumineszenzspektroskopie mit zirkularer Polarisation -- Nukleotide -- Zink; Chemistry -- Periodicals; 540
• DOI: 10.1002/ange.201801248
• Languages: English
• ISSNs: 0044-8249

Abstract

Abstract: A series of three europium complexes bearing picolyl amine moieties was found to possess differing binding affinities towards Zn 2+ and three nucleotides: AMP, ADP, and ATP. A large increase in the total emission intensity was observed upon binding Zn 2+, followed by signal amplification upon the addition of nucleotides. The resulting adducts possessed strong induced circularly polarised emission, with ADP and ATP signals of opposite sign. Model DFT geometries of the adducts suggest the Δ diastereoisomer is preferred for ATP and the Λ isomer for ADP/AMP. This change in sign allows the ADP/ATP (or AMP/ATP) ratio to be assessed by monitoring changes in the emission dissymmetry factor, g em .