Transcriptome analysis of PCOS arrested 2-cell embryos. Issue 8 (18th April 2018)

• Record Type: Journal Article
• Authors: Lu, Cuiling; Chi, Hongbin; Wang, Yapeng; Feng, Xue; Wang, Lina; Huang, Shuo; Yan, Liying; Lin, Shengli; Liu, Ping; Qiao, Jie
• Is Part Of: Cell cycle. Volume 17:Issue 8(2018)
• Page Start: 1007
• Page End: 1013
• Publication Date: 2018-04-18
• Subjects: PCOS -- embryo arrest -- transcriptome; Cell cycle -- Periodicals; 571.84377
• DOI: 10.1080/15384101.2018.1467678
• Languages: English
• ISSNs: 1538-4101

Abstract

ABSTRACT: In an attempt to explore the early developmental arrest in embryos from polycystic ovarian syndrome (PCOS) patients, we sequenced the transcriptome profiles of PCOS arrested 2-cell embryos, non-PCOS arrested 2-cell embryos and non-arrested 2-cell embryos using single-cell RNA-Seq technique. Differential expression analysis was performed using the DEGSeq R package. Gene Ontology (GO) enrichment was analyzed using the GOseq R package. Data revealed 62 differentially expressed genes between non-PCOS arrested and PCOS arrested embryos and 2217 differentially expressed genes between PCOS arrested and non-arrested 2-cell embryos. A total of 49 differently expressed genes (DEGs) were annotated with GO terms in the up-regulated genes between PCOS arrested and non-PCOS arrested embryos after GO enrichment. A total of 29 DEGs were annotated with GO terms in the down-regulated genes between PCOS arrested and non-arrested 2-cell embryos after GO enrichment. These data can provide a reference for screening specific genes involved in the arrest of PCOS embryos.