Biostimulation and bioaugmentation of native microbial community accelerated bioremediation of oil refinery sludge. (April 2018)

• Record Type: Journal Article
• Authors: Roy, Ajoy; Dutta, Avishek; Pal, Siddhartha; Gupta, Abhishek; Sarkar, Jayeeta; Chatterjee, Ananya; Saha, Anumeha; Sarkar, Poulomi; Sar, Pinaki; Kazy, Sufia K.
• Is Part Of: Bioresource technology. Volume 253(2018)
• Page Start: 22
• Page End: 32
• Publication Date: 2018-04
• Subjects: Biostimulation -- Bioaugmentation -- Bioremediation -- Total petroleum hydrocarbon (TPH) -- Microbial community; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2018.01.004
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Native microbes based engineered system enhanced refinery sludge bioremediation. Combined biostimulation-bioaugmentation strategy resulted in maximum remediation. Bioremediation enhanced diverse groups of microorganisms. Nutrient-induced community dynamics was driving force for accelerated remediation. Abstract: Scope for developing an engineered bioremediation strategy for the treatment of hydrocarbon-rich petroleum refinery waste was investigated through biostimulation and bioaugmentation approaches. Enhanced (46–55%) total petroleum hydrocarbon (TPH) attenuation was achieved through phosphate, nitrate or nitrate+phosphate amendment in the sludge with increased (upto 12%) abundance of fermentative, hydrocarbon degrading, sulfate-reducing, CO2 -assimilating and methanogenic microorganisms ( Bacillus, Coprothermobacter, Rhodobacter, Pseudomonas, Achromobacter, Desulfitobacter, Desulfosporosinus, T78, Methanobacterium, Methanosaeta, etc). Together with nutrients, bioaugmentation with biosurfactant producing and hydrocarbon utilizing indigenous Bacillus strains resulted in 57–75% TPH reduction. Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) analysis revealed enhanced gene allocation for transporters (0.45–3.07%), ABC transporters (0.38–2.07%), methane (0.16–1.06%), fatty acid (0.018–0.15%), nitrogen (0.07–0.17%), butanoate (0.06–0.35%), propanoate (0.004–0.26%) metabolism and some xenobiotics (0.007–0.13%) degradation. This study indicated that nutrient-induced community dynamics of native microorganisms and their metabolic interplay within oil refinery sludge could be a driving force behind accelerated bioremediation.