Mechanisms of noradrenergic modulation of synaptic transmission and neuronal excitability in ventral horn neurons of the rat spinal cord. (1st June 2019)

• Record Type: Journal Article
• Authors: Shoji, Hirokazu; Ohashi, Masayuki; Hirano, Toru; Watanabe, Kei; Endo, Naoto; Baba, Hiroshi; Kohno, Tatsuro
• Is Part Of: Neuroscience. Volume 408(2019)
• Page Start: 161
• Page End: 176
• Publication Date: 2019-06-01
• Subjects: monoamine -- adrenoceptor -- ventral horn neuron -- spinal cord injury -- patch clamp; ACSF artificial cerebrospinal fluid -- AP action potential -- EPSC excitatory postsynaptic current -- GABA γ-aminobutyric acid -- IPSC inhibitory postsynaptic current -- IR-DIC infrared-differential interference contrast -- NA noradrenaline -- mEPSC miniature EPSC -- mIPSC miniature IPSC -- RMP resting membrane potential -- SCI spinal cord injury -- sEPSC spontaneous EPSC -- sIPSC spontaneous IPSC -- TEA tetraethylammonium -- TTX tetrodotoxin -- VH ventral horn; Neurochemistry -- Periodicals; Neurophysiology -- Periodicals; Neurology -- Periodicals; Neurochimie -- Périodiques; Neurophysiologie -- Périodiques; Neurochemistry; Neurophysiology; Electronic journals; Periodicals; Electronic journals; 612.8
• DOI: 10.1016/j.neuroscience.2019.03.026
• Languages: English
• ISSNs: 0306-4522

Abstract

Abstract: Noradrenaline (NA) modulates the spinal motor networks for locomotion and facilitates neuroplasticity, possibly assisting neuronal network activation and neuroplasticity in the recovery phase of spinal cord injuries. However, neither the effects nor the mechanisms of NA on synaptic transmission and neuronal excitability in spinal ventral horn (VH) neurons are well characterized, especially in rats aged 7 postnatal days or older. To gain insight into NA regulation of VH neuronal activity, we used a whole-cell patch-clamp approach in late neonatal rats (postnatal day 7–15). In voltage-clamp recordings at − 70 mV, NA increased the frequency and amplitude of excitatory postsynaptic currents via the activation of somatic α1 - and β-adrenoceptors of presynaptic neurons. Moreover, NA induced an inward current through the activation of postsynapticα1 - and β-adrenoceptors. At a holding potential of 0 mV, NA also increased frequency and amplitude of both GABAergic and glycinergic inhibitory postsynaptic currents via the activation of somatic adrenoceptors in presynaptic neurons. In current-clamp recordings, NA depolarized resting membrane potentials and increased the firing frequency of action potentials in VH neurons, indicating that it enhances the excitability of these neurons. Our findings provide new insights that establish NA-based pharmacological therapy as an effective method to activate neuronal networks of the spinal VH in the recovery phase of spinal cord injuries. Highlights: The effects of noradrenaline (NA) on ventral horn neurons of rats were examined using a whole-cell patch-clamp technique. NA facilitated excitatory synaptic transmission via the activation of α1 - and β-adrenoceptors in presynaptic neurons. NA induced an inward current via the activation of postsynaptic α1 - and β-adrenoceptors. NA enhanced inhibitory synaptic transmission via the activation of somatic adrenoceptors in presynaptic neurons. NA depolarized the resting membrane potential and increased the firing frequency of action potentials.