A novel strategy for the efficient removal of toxic cyanate by the combinatorial use of recombinant enzymes immobilized on aminosilane modified magnetic nanoparticles. (April 2018)

• Record Type: Journal Article
• Authors: Ranjan, Bibhuti; Pillai, Santhosh; Permaul, Kugenthiren; Singh, Suren
• Is Part Of: Bioresource technology. Volume 253(2018)
• Page Start: 105
• Page End: 111
• Publication Date: 2018-04
• Subjects: Cyanate -- Carbonic anhydrase -- Cyanase -- Wastewater -- Magnetic nanoparticles; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2017.12.087
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: First report on the combinatorial use of rTl-Cyn and rTl-CA for the efficient removal of cyanate. First report on the co-immobilization of rTl-Cyn and rTl-CA onto magnetic nanoparticles. Co-immobilized enzymes showed 80% less dependence on bicarbonate compared to rTl-Cyn alone. Co-immobilized enzymes displayed >90% of initial activity after ten cycles. Abstract: Cyanase detoxifies cyanate by transforming it to ammonia and carbon dioxide in a bicarbonate-dependent reaction, however, dependence on bicarbonate limits its utilization in large-scale applications. A novel strategy was therefore developed for overcoming this bottleneck by the combined application of cyanase (rTl-Cyn) and carbonic anhydrase (rTl-CA). The synergistic effect of rTl-Cyn and rTl-CA could reduce the dependence of bicarbonate by 80%, compared to using rTl-Cyn alone. Complete degradation of cyanate (4 mM) was achieved with buffered conditions and 85 ± 5% degradation with industrial wastewater sample, when 20 U of rTl-Cyn was applied. Furthermore, a similar percentage of degradation was achieved using 80% less bicarbonate, when rTl-Cyn and rTl-CA were used together under identical conditions. In addition, rTl-Cyn and rTl-CA were immobilized onto the magnetic nanoparticles and their catalytic activity, stability and reusability were also evaluated. This is the first report on the synergistic biocatalysis by rTl-Cyn and rTl-CA, for cyanate detoxification.