Culture conditions and nutrition requirements for the mycelial growth of Isaria farinosa (Hypocreales: Cordycipitaceae) and the altitude effect on its growth and metabolome. Issue 1 (December 2018)

• Record Type: Journal Article
• Authors: Liu, Fei; Xiang, Meichun; Guo, Yanlei; Wu, Xiaoli; Lu, Guangxin; Yang, Yong; Liu, Xingzhong; Chen, Shijiang; Zhang, Guozhen; Shi, Wangpeng
• Is Part Of: Scientific reports. Volume 8:Issue 1(2018)
• Page Start: 1
• Page End: 15
• Publication Date: 2018-12
• Subjects: Natural history -- Research -- Periodicals; Biology -- Research -- Periodicals; Physical sciences -- Research -- Periodicals; Earth sciences -- Research -- Periodicals; Environmental sciences -- Research -- Periodicals; 502.85
• DOI: 10.1038/s41598-018-33965-z
• Languages: English
• ISSNs: 2045-2322

Abstract

Abstract Isaria farinosa is a pathogen of alpineThitarodes larvae that are hosts for the Chinese medicinal fungus, Ophiocordyceps sinensis . A matrix analysis indicated that the optimal culture conditions for the mycelial growth ofI .farinosa are a 50-mL liquid broth in a 250-mL flask at more than 100-rpm rotation and 15–25 °C. Illumination does not affect the mycelial growth. The optimal nutrition requirements are D-(+)-galactose and D-(−)-fructose as carbon resources and D-cysteine as well as yeast powder, peptone, and beef extract as nitrogen resources at a carbon-to-nitrogen ratio of 1:1 to 1:7. The mineral component and vitamins also significantly increase the mycelial growth ofI .farinosa . Based on the optimal culture conditions and nutrition requirements for the mycelial growth ofI .farinosa, the effects of altitude on mycelial growth and its metabolome were evaluated using quadrupole-time-of-flight/mass spectrometry, principal component analysis and partial least squares discriminant analysis. The altitude did not affect the mycelial production but significantly regulated its metabolome. The study presents a new approach to better select a method for producing more useful metabolites and highlights the necessity of establishing standards for culturing methods related to altitude to preserve fungal quality; additionally, the results indicate that the use of a fermenter may meet the demands of large-scale mycelial production.