An interspecies barrier to tetraploid complementation and chimera formation. Issue 1 (December 2018)

• Record Type: Journal Article
• Authors: Yamaguchi, Tomoyuki; Sato, Hideyuki; Kobayashi, Toshihiro; Kato-itoh, Megumi; Goto, Teppei; Hara, Hiromasa; Mizuno, Naoaki; Yanagida, Ayaka; Umino, Ayumi; Hamanaka, Sanae; Suchy, Fabian; Masaki, Hideki; Ota, Yasunori; Hirabayashi, Masumi; Nakauchi, Hiromitsu
• Is Part Of: Scientific reports. Volume 8:Issue 1(2018)
• Page Start: 1
• Page End: 10
• Publication Date: 2018-12
• Subjects: Natural history -- Research -- Periodicals; Biology -- Research -- Periodicals; Physical sciences -- Research -- Periodicals; Earth sciences -- Research -- Periodicals; Environmental sciences -- Research -- Periodicals; 502.85
• DOI: 10.1038/s41598-018-33690-7
• Languages: English
• ISSNs: 2045-2322

Abstract

Abstract To study development of the conceptus in xenogeneic environments, we assessed interspecies chimera formation as well as tetraploid complementation between mouse and rat. Overall contribution of donor PSC-derived cells was lower in interspecies chimeras than in intraspecies chimeras, and high donor chimerism was associated with anomalies or embryonic death. Organ to organ variation in donor chimerism was greater in interspecies chimeras than in intraspecies chimeras, suggesting species-specific affinity differences among interacting molecules necessary for organogenesis. In interspecies tetraploid complementation, embryo development was near normal until the stage of placental formation, after which no embryos survived.