Changes in apoptosis, proliferation and T lymphocyte subtype on thymic cells of SPF chickens infected with reticuloendotheliosis virus. (July 2019)

• Record Type: Journal Article
• Authors: Fu, Lisheng; Wang, Xiaoyan; Zhai, Jie; Qi, Wei; Jing, Long; Ge, Yiyang; Gao, XueLi; Liu, Chaonan; Lv, Xiaoping; Zheng, Shimin
• Is Part Of: Molecular immunology. Volume 111(2019:Jul.)
• Page Start: 87
• Page End: 94
• Publication Date: 2019-07
• Subjects: Reticuloendotheliosis -- Virus -- Immunosuppression -- T cell subtype -- Apoptosis -- Proliferation; Immunochemistry -- Periodicals; Molecular biology -- Periodicals; Immunochemistry -- Periodicals; Allergy and Immunology -- Periodicals; Molecular Biology -- Periodicals; Immunochimie -- Périodiques; Biologie moléculaire -- Périodiques; Immunochemistry; Molecular biology; Periodicals; Electronic journals; 571.96
• DOI: 10.1016/j.molimm.2019.04.003
• Languages: English
• ISSNs: 0161-5890

Abstract

Highlights: Successfully applied the mode for reticuloendotheliosis viral (REV) infection in SPF chickens and assessed the replication ability of the REV gene in thymic lymphocytes cells. Apoptosis and the inhibition of lymphocyte proliferation lead to atrophy of the thymus and decrease in the number of lymphocytes. REV infection causes decrease in the number of thymic lymphocytes, changes T cell subsets and enhances the immunosuppressive effect. Abstract: Reticuloendotheliosis virus (REV), an avian retrovirus is able to infect a variety of birds and can cause immunosuppression. The aim of this study was to investigate the relationship of thymic lymphocytes apoptosis, proliferation and T cell subtype with immunosuppression. In this study, a hundred and twenty one-day old SPF chickens were randomly divided into control groups (group C) and a REV infection groups (group I). The chickens of group I received intraperitoneal injections of REV with 10 4.62 /0.1 ml TCID50 . On day 14, 21, 28 and 35 post-inoculation, the chickens of C group and I group were sacrificed by cardiac puncture blood collection, and the thymic lymphocytes was sterile collected. The proliferation ability of lymphocytes was tested by Cell Counting Kit-8. Flow cytometry was performed to detect apoptosis, cell cycle stage and the change in T cell subtype. The RNA genome copy numbers of REV virus were detected using real-time PCR. Real-time PCR and western blotting were performed to analyze the expression of CyclinD1 and Bcl-2. Our results showed that REV genome copy number steadily declined, the proliferation potential of thymic lymphocytes was inhibited, lymphocytes apoptosed, the ratio of CD4+/CD8+ decreased and the expression of CyclinD1 and Bcl-2 were firstly inhibited, then rapidly recovered. Thus, immunosuppression lead by REV is closely related to the change of T cell subtype, apoptosis, and proliferation of thymic lymphocytes.