Semi‐quantitative duplex RT‐PCR reveals the low occurrence of Porcine Pegivirus and Atypical Porcine Pestivirus in diagnostic samples from the United States. Issue 3 (21st March 2019)

• Record Type: Journal Article
• Authors: Chen, Fangzhou; Knutson, Todd P.; Braun, Eli; Jiang, Yin; Rossow, Stephanie; Marthaler, Douglas G.
• Is Part Of: Transboundary and emerging diseases. Volume 66:Issue 3(2019)
• Page Start: 1420
• Page End: 1425
• Publication Date: 2019-03-21
• Subjects: atypical porcine pestivirus -- Pegivirus -- phylogenetics; Veterinary medicine -- Periodicals; 636.089
• DOI: 10.1111/tbed.13154
• Languages: English
• ISSNs: 1865-1674

Abstract

Summary: Porcine Pegivirus (PPgV) and Atypical Porcine Pestivirus (APPV) are two recently identified porcine viruses. In this study, the identification of two viruses by metagenomic sequencing, and a duplex semi‐quantitative RT‐PCR was developed to detect these pathogens simultaneously. The PPgV strain Minnesota‐1/2016 had a 95.5%–96.3% nucleotide identity and clustered with the recently identified US PPgV strains, which is a distant clade from the German PPgV strains. The APPV strain Minnesota‐1/2016 shared an 87.3%–92.0% nucleotide identity with the other global APPV strains identity but only shared an 82.8%–83.0% nucleotide identity with clade II consisting of strain identified in China. Detection of both PPgV and APPV was 9.0% of the diagnostic cases. Co‐infection of PPgV and APPV was identified in 7.5% of the diagnostic cases. The occurrence and genetic characterization of PPgV and APPV further enhance our knowledge regarding these new pathogens in the United States.