Electrospun microporous gelatin–polycaprolactone blend tubular scaffold as a potential vascular biomaterial. Issue 7 (3rd May 2019)

• Record Type: Journal Article
• Authors: Joy, Jincy; Pereira, Jessica; Aid‐Launais, Rachida; Pavon‐Djavid, Graciela; Ray, Alok R; Letourneur, Didier; Meddahi‐Pellé, Anne; Gupta, Bhuvanesh
• Is Part Of: Polymer international. Volume 68:Issue 7(2019)
• Page Start: 1367
• Page End: 1377
• Publication Date: 2019-05-03
• Subjects: gelatin–polycaprolactone blend -- electrospinning -- microfibrous tubular constructs -- vascular biomaterial; Plastics -- Periodicals; Polymers -- Periodicals; Polymerization -- Periodicals; 547.7
• DOI: 10.1002/pi.5827
• Languages: English
• ISSNs: 0959-8103

Abstract

Abstract: The work reported involved the fabrication of an electrospun tubular conduit of a gelatin and polycaprolactone (PCL) blend as an adventitia‐equivalent construct. Gelatin was included as the matrix for increased biocompatibility with the addition of PCL for durability. This is contrary to most of the literature available for biomaterials based on blends of gelatin and PCL where PCL is the major matrix. The work includes the assiduous selection of key electrospinning parameters to obtain smooth bead‐free fibres with a narrow distribution of pore size and fibre diameter. Few reports elucidate the optimization of all electrospinning parameters to fabricate tubular conduits with a focus on obtaining homogeneous pores and fibres. This stepwise investigation would be unique for the fabrication of gelatin–PCL electrospun tubular constructs. The fabricated microfibrous gelatin–PCL constructs had pores of size ca 50–100 μm reportedly conducive for cell infiltration. The measured value of surface roughness of 57.99 ± 17.4 nm is reported to be favourable for protein adhesion and cell adhesion. The elastic modulus was observed to be similar to that of the tunica adventitia of the native artery. Preliminary in vitro and in vivo biocompatibility tests suggest safe applicability as a biomaterial. Minimal cytotoxicity was observed using MTT assay. Subcutaneous implantation of the scaffold demonstrated acute inflammation which decreased by day 15. The findings of this study could enable the fabrication of smooth bead‐free microfibrous gelatin–PCL tubular construct as viable biomaterial which can be included in a bilayer or a trilayer scaffold for vascular tissue engineering. © 2019 Society of Chemical Industry Abstract : Fabrication of an electrospun tubular construct based on a gelatin–polycaprolactone blend using optimized electrospinning parameters led to a microporous conduit. The physiological properties and biocompatibility evaluation validated the construct as a vascular biomaterial.