Searching for calcium antagonists for hypertension disease therapy from Moutan Cortex, using bioactivity integrated UHPLC‐QTOF‐MS. Issue 4 (11th March 2019)

• Record Type: Journal Article
• Authors: Lu, Yujie; Deng, Yanfang; Liu, Wenjuan; Jiang, Min; Bai, Gang
• Is Part Of: Phytochemical analysis. Volume 30:Issue 4(2019)
• Page Start: 456
• Page End: 463
• Publication Date: 2019-03-11
• Subjects: bioactivity‐integrated UHPLC‐QTOF -- calcium antagonists -- inositol 1, 4, 5‐trisphosphate receptors -- Moutan Cortex -- voltage‐operated Ca2+ channels; Plants -- Analysis -- Periodicals; Plants -- chemistry -- Periodicals; 572.2
• DOI: 10.1002/pca.2828
• Languages: English
• ISSNs: 0958-0344

Abstract

Abstract: Introduction: Calcium channel blockers (CCBs) are currently the most commonly used drugs for the treatment of hypertension. Moutan Cortex (MC), a traditional Chinese herb, has been found to have an anti‐hypertensive effect. However, its potential mechanisms in the regulation of intracellular calcium concentration ([Ca 2+ ]i ) remain poorly understood. Objective: The main objective of this work was to identify the potential calcium antagonists from MC and study their molecular mechanisms. Methods: Ultra‐high performance liquid chromatography‐quadrupole‐time‐of‐fight‐mass spectrometry (UHPLC‐QTOF‐MS) analysis combined with a dual‐luciferase reporter assay was utilised to systematically screen the calcium antagonistic active ingredients in the methanol extract of MC. Additionally, the molecular mechanism of these compounds was further studied using live‐cell imaging analysis with the calcium ion (Ca 2+ ) probe dye fluo‐4/AM to monitor changes in [Ca 2+ ]i . Results: Three monoterpenoids (paeoniflorin, benzoylpaeoniflorin and mudanpioside C), one phenolic acid (paeonol) and one gallotannin (1, 2, 3, 4, 6‐ O ‐pentagalloylglucose) were screened out as potential calcium antagonists in MC. Among them, the calcium antagonistic activity of benzoylpaeoniflorin, mudanpioside C and 1, 2, 3, 4, 6‐ O ‐pentagalloylglucose is first reported. Additionally, paeoniflorin, benzoylpaeoniflorin, mudanpioside C and paeonol can effectively block voltage‐operated Ca 2+ channels (VOCCs) to exert calcium antagonism, while 1, 2, 3, 4, 6‐ O ‐pentagalloylglucose plays a role in blocking inositol 1, 4, 5‐trisphosphate receptors (IP3Rs). Conclusion: This work indicated that the anti‐hypertensive efficacy of MC acted through multiple components selectively antagonising multiple cell signalling pathways to regulate [Ca 2+ ]i . Furthermore, they could be considered as a reference standard for controlling the quality of Chinese medicinal materials. Abstract : MC contains five potential calcium antagonists acting on multi-signaling pathways. Paeoniflorin, benzoylpaeoniflorin, mudanpioside C and paeonol effectively block VOCCs. 1, 2, 3, 4, 6‐O‐Pentagalloylglucose plays a role in blocking IP3Rs.