Structural characterisation and ACE-inhibitory activities of polysaccharide from Gastrodia elata Blume. Issue 12 (18th June 2019)

• Record Type: Journal Article
• Authors: Zhu, Zhen-Yuan; Chen, Chun-Juan; Sun, Hui-Qing; Chen, Li-Jing
• Is Part Of: Natural product research. Volume 33:Issue 12(2019)
• Page Start: 1721
• Page End: 1726
• Publication Date: 2019-06-18
• Subjects: Structural properties -- polysaccharide -- Gastrodia elata Blume; Natural products -- Periodicals; Natural products -- Research -- Periodicals; Biological Factors -- Periodicals; Biological Products -- Periodicals; 547
• DOI: 10.1080/14786419.2018.1434643
• Languages: English
• ISSNs: 1478-6419

Abstract

Abstract: The structural properties and Angiotensin-I converting enzyme (ACE) inhibition activities of a polysaccharide (PGE) extracted from Gastrodia elata Blume were investigated. PGE was extracted using hot water and purified by Sephadex G-200 followed by ultra-filtration. The structural characterisation of PGE was analysed by FT–IR, NMR spectroscopy, specific rotation determination, periodate oxidation-smith degradation, methylation analysis, GC–MS and Congo red test. The results revealed that PGE was composed by glucose, with an average molecular weight of 1.54 × 10 3 kDa. The structure of PGE was 1→3 and 1→4, 6-branched-glucopyranose that had a linear backbone of (1 → 4)-linked-d -glucopyranose (Glcp). ACE-inhibitory activity results showed that PGE was efficient to inhibit ACE and the IC50 value was 0.66 mg/mL. Abstract :