A Sensitive IHC Method for Monitoring Autophagy-Specific Markers in Human Tumor Xenografts. (10th May 2016)

• Record Type: Journal Article
• Authors: He, Helen; Yang, Yu; Xiang, Zhongmin; Yu, Lunyin; Chouitar, Jouhara; Yu, Jie; D'Amore, Natalie Roy; Li, Ping; Li, Zhi; Bowman, Douglas; Theisen, Matthew; Brownell, James E.; Tirrell, Stephen
• Other Names: Chung Yuen-Li Academic Editor.
• Is Part Of: Journal of biomarkers. Volume 2016(2016)
• Publication Date: 2016-05-10
• Subjects: Biochemical markers -- Periodicals; Biomarkers; Biochemical markers; Periodicals; Electronic journals; Periodicals; Electronic journals; 611.01816
• DOI: 10.1155/2016/1274603
• Languages: English
• ISSNs: 2090-8660

Abstract

Abstract : Objective. Use of tyramide signal amplification (TSA) to detect autophagy biomarkers in formalin fixed and paraffin embedded (FFPE) xenograft tissue. Materials and Methods. Autophagy marker regulation was studied in xenograft tissues using Amp HQ IHC and standard IHC methods. Results. The data demonstrate the feasibility of using high sensitivity TSA IHC assays to measure low abundant autophagy markers in FFPE xenograft tissue.