Stress granules counteract senescence by sequestration of PAI‐1. (29th March 2018) - Omer, Amr
Abstract: Cellular senescence is a physiological response by which an organism halts the proliferation of potentially harmful and damaged cells. However, the accumulation of senescent cells over time can become deleterious leading to diseases and physiological decline. Our data reveal a novel interplay between senescence and the stress response that affects both the progression of senescence and the behavior of senescent cells. We show that constitutive exposure to stress induces the formation of stress granules (SGs) in proliferative and presenescent cells, but not in fully senescent cells. Stress granule assembly alone is sufficient to decrease the number of senescent cells without affecting the expression of bona fide senescence markers. SG‐mediated inhibition of senescence is associated with the recruitment of the plasminogen activator inhibitor‐1 (PAI‐1), a known promoter of senescence, to these entities. PAI‐1 localization to SGs increases the translocation of cyclin D1 to the nucleus, promotes RB phosphorylation, and maintains a proliferative, non‐senescent state. Together, our data indicate that SGs may be targets of intervention to modulate senescence in order to impair or prevent its deleterious effects. Synopsis: Stress granules counteract senescence by sequestration of PAI‐1, which activates the cyclin D1/Rb pathway and promotes cell proliferation. In cells undergoing senescence, chronic stress induces stress granule (SG) formation during the early stages but not in fully senescent cells. SG assembly decreases cellular senescence. The recruitment of PAI‐1, an established promoter of senescence, to SGs reduces its secretion and cell commitment to senescence through activation of the cyclin D1/Rb signalling pathway. Abstract : Stress granules counteract senescence by sequestration of PAI‐1, which activates the cyclin D1/Rb pathway and promotes cell proliferation.