Zinc Oxide Nanoparticles and Voltage‐Gated Human Kv11.1 Potassium Channels Interact through a Novel Mechanism. Issue 15 (26th February 2018)

• Record Type: Journal Article
• Authors: Piscopo, Stefania; Brown, Euan R.
• Is Part Of: Small. Volume 14:Issue 15(2018)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2018-02-26
• Subjects: channel gating -- hERG potassium channel -- lipid bilayer -- nanoparticles -- zinc oxide; Nanotechnology -- Periodicals; Nanoparticles -- Periodicals; Microtechnology -- Periodicals; 620.5
• DOI: 10.1002/smll.201703403
• Languages: English
• ISSNs: 1613-6810

Abstract

Abstract: Membrane–nanoparticle interactions are important in determining the effects of manufactured nanomaterials on cell physiology and pathology. Here, silica, titanium, zinc, and magnesium oxide nanoparticles are screened against human hERG (Kv 11.1) voltage‐gated potassium channels under a whole‐cell voltage clamp. 10 µg mL −1 ZnO uniquely increases the amplitude of the steady‐state current, decreases the rate of hERG current inactivation during steady‐state depolarization, accelerates channel deactivation during resurgent tail currents, and shows no significant alteration of current activation rate or voltage dependence. In contrast, ZnCl2 causes increased current suppression with increasing concentration and fails to replicate the nanoparticle effect on decreasing inactivation. The results show a novel class of nanoparticle–biomembrane interaction involving channel gating rather than channel block, and have implications for the use of nanoparticles in biomedicine, drug delivery applications, and nanotoxicology. Abstract : An electrophysiological screening of silica, titanium, zinc, and magnesium oxide nanoparticles against human hERG (Kv 11.1) voltage‐gated potassium channels finds that zinc oxide nanoparticles uniquely alter channel gating. The results reveal a novel class of nanoparticle–biomembrane interaction involving channel gating rather than channel block, and have implications for the use of ZnO nanoparticles in biomedicine, drug delivery applications, and nanotoxicology.