Characterization of a novel HLA‐B*39:01:01‐related allele, HLA‐B*39:130, by cloning and phasing. (30th October 2017)

• Record Type: Journal Article
• Authors: Li, L. X.; Tian, W.; Zhu, F. M.; Wang, W. Y.; Cai, J. H.
• Is Part Of: International journal of immunogenetics. Volume 44:Number 6(2017:Dec.)
• Page Start: 356
• Page End: 358
• Publication Date: 2017-10-30
• Subjects: cloning -- HLA‐B*39:130 -- phasing -- sequence‐based typing -- southern Chinese Han population; Immunogenetics -- Periodicals; 571.9648
• DOI: 10.1111/iji.12345
• Languages: English
• ISSNs: 1744-3121

Abstract

Summary: A novel HLA‐B*39:01:01 ‐related variant, HLA‐B*39:130, has been identified in a normal individual of Han ethnicity in Hunan province, southern China. Following Sanger polymerase chain reaction–sequence‐based typing (PCR‐SBT), this new allele was further confirmed by cloning, phasing and sequencing. Aligned with HLA‐B*39:01:01, HLA‐B*39:130 has a nonsynonymous thymine substitution at nucleotide position 94 in exon 4, resulting in amino acid change from threonine to isoleucine at codon 214 (ACA→ATA) of the mature HLA‐B mRNA molecule.