Hybrid Indicators for Fast and Sensitive Voltage Imaging. (8th March 2018)

• Record Type: Journal Article
• Authors: Xu, Yongxian; Peng, Luxin; Wang, Sicong; Wang, Anqi; Ma, Ruirui; Zhou, Ying; Yang, Jiahe; Sun, De‐en; Lin, Wei; Chen, Xing; Zou, Peng
• Is Part Of: Angewandte Chemie. Volume 130:Number 15(2018)
• Page Start: 4013
• Page End: 4017
• Publication Date: 2018-03-08
• Subjects: Fluoreszenzsonden -- FRET -- Klick-Chemie -- Protein-Engineering -- Spannungsbasierte Bildgebung; Chemistry -- Periodicals; 540
• DOI: 10.1002/ange.201712614
• Languages: English
• ISSNs: 0044-8249

Abstract

Abstract: Membrane voltage is an important biophysical signal that underlies intercellular electrical communications. A fluorescent voltage indicator is presented that enables the investigation of electrical signaling at high spatial resolution. The method is built upon the site‐specific modification of microbial rhodopsin proteins with organic fluorophores, resulting in a hybrid indicator scaffold that is one of the most sensitive and fastest orange‐colored voltage indicators developed to date. We applied this technique to optically map electrical connectivity in cultured cells, which revealed gap junction‐mediated long‐range coupling that spanned over hundreds of micrometers.