Cytotoxicity of Cricula triphenestrata Cocoon Extract on Human Fibroblasts. (9th July 2012)

• Record Type: Journal Article
• Authors: Sunarintyas, Siti; Siswomihardjo, Widowati; Tontowi, Alva Edy
• Other Names: Yamaguchi Masaru Academic Editor.
• Is Part Of: International journal of biomaterials. Volume 2012(2012)
• Publication Date: 2012-07-09
• Subjects: Biomedical materials -- Periodicals; Biomedical and Dental Materials; Biomedical materials; Electronic journals; Periodicals; Fulltext; Internet Resources; Periodicals; Periodicals; 610.28
• DOI: 10.1155/2012/493075
• Languages: English
• ISSNs: 1687-8787

Abstract

Abstract : Objectives . The aim of this paper was to evaluate the cytotoxicity of Indonesian silkworm cocoon extract of Cricula triphenestrata on human fibroblasts. Methods and Materials . The cocoon shells of the silkworm Cricula triphenestrata were degumming. The shells were mixed with an aqueous solution of 0.3% Na2 CO3 at 98°C for 1 hour. The solution was then dialyzed in cellulose membranes against deionized water for 3 days. The cocoon shells extract powder was collected via rotary evaporation and dried under freeze dryer. Cell culture medium was exposed to Cricula triphenestrata cocoon extract (0.01–100 μ g/mL) for 24 hours. The primary human gingival fibroblasts were exposed to the treated cell culture medium for 24 hours. Cytotoxicity evaluation was done by MTT method. The data were analyzed by one-way ANOVA. Result . The result revealed no significant cytotoxicity of Cricula triphenestrata cocoon extract against human fibroblasts at a concentration up to 100 μ g/mL (P > 0.05 ). Conclusion . Cricula triphenestrata cocoon extract was not cytotoxic on human gingival fibroblast cells.