HKOH‐1: A Highly Sensitive and Selective Fluorescent Probe for Detecting Endogenous Hydroxyl Radicals in Living Cells. (14th September 2017)

• Record Type: Journal Article
• Authors: Bai, Xiaoyu; Huang, Yueyang; Lu, Mingyang; Yang, Dan
• Is Part Of: Angewandte Chemie. Volume 129:Number 42(2017)
• Page Start: 13053
• Page End: 13057
• Publication Date: 2017-09-14
• Subjects: Biologische Bildgebung -- Fluoreszenzsonden -- Flusszytometrie -- Hydroxylradikale -- Lebende Zellen; Chemistry -- Periodicals; 540
• DOI: 10.1002/ange.201705873
• Languages: English
• ISSNs: 0044-8249

Abstract

Abstract: The hydroxyl radical ( . OH), one of the most reactive and deleterious reactive oxygen species (ROS), has been suggested to play an essential role in many physiological and pathological scenarios. However, a reliable and robust method to detect endogenous . OH is currently lacking owing to its extremely high reactivity and short lifetime. Herein we report a fluorescent probe HKOH‐1 with superior in vitro selectivity and sensitivity towards . OH. With this probe, we have calibrated and quantified the scavenging capacities of a wide range of reported . OH scavengers. Furthermore, HKOH‐1r, which was designed for better cellular uptake and retention, has performed robustly in detection of endogenous . OH generation by both confocal imaging and flow cytometry. Furthermore, this probe has been applied to monitor . OH generation in HeLa cells in response to UV light irradiation. Therefore, HKOH‐1 could be used for elucidating . OH related biological functions.