Bacteriocin‐like inhibitory activities of seven Lactobacillus delbrueckii subsp. bulgaricus strains against antibiotic susceptible and resistant Helicobacter pylori strains. (23rd October 2017)

• Record Type: Journal Article
• Authors: Boyanova, L.; Gergova, G.; Markovska, R.; Yordanov, D.; Mitov, I.
• Is Part Of: Letters in applied microbiology. Volume 65:Number 6(2017:Dec.)
• Page Start: 469
• Page End: 474
• Publication Date: 2017-10-23
• Subjects: antibiotics -- bacteriocins -- Helicobacter -- Lactobacillus -- probiotics; Microbiology -- Periodicals; 660.62
• DOI: 10.1111/lam.12807
• Languages: English
• ISSNs: 0266-8254

Abstract

Abstract: The aim of the study was to detect anti‐ Helicobacter pylori activity of seven Lactobacillus delbrueckii subsp. bulgaricus (GLB) strains by four cell‐free supernatant (CFS) types. Activity of non‐neutralized and non‐heat‐treated (CFSs1), non‐neutralized and heat‐treated (CFSs2), pH neutralized, catalase‐treated and non‐heat‐treated (CFSs3), or neutralized, catalase‐ and heat‐treated (CFSs4) CFSs against 18 H. pylori strains (11 of which with antibiotic resistance) was evaluated. All GLB strains produced bacteriocin‐like inhibitory substances (BLISs), the neutralized CFSs of two GLB strains inhibited >81% of test strains and those of four GLB strains were active against >71% of antibiotic resistant strains. Two H. pylori strains were BLIS resistant. The heating did not reduce the CFS activity. Briefly, all GLB strains evaluated produced heat‐stable BLISs, although GLB and H. pylori strain susceptibility patterns exhibited differences. Bacteriocin‐like inhibitory substance activity can be an advantage for the probiotic choice for H. pylori infection control. Significance and Impact of the Study: In this study, anti‐ Helicobacter pylori activity of seven Lactobacillus delbrueckii subsp. bulgaricus (GLB) strains was evaluated by four cell‐free supernatant (CFS) types. The GLB strains produced heat‐stable bacteriocin‐like inhibitory substances (BLISs) with a strong anti‐ H. pylori activity and some neutralized, catalase‐ and heat‐treated CFSs inhibited >83% of the test strains. Bacteriocin‐like inhibitory substance production of GLB strains can render them valuable probiotics in the control of H. pylori infection. Abstract : Significance and Impact of the Study: In this study, anti‐ Helicobacter pylori activity of seven Lactobacillus delbrueckii subsp. bulgaricus (GLB) strains was evaluated by four cell‐free supernatant (CFS) types. The GLB strains produced heat‐stable bacteriocin‐like inhibitory substances (BLISs) with a strong anti‐ H. pylori activity and some neutralized, catalase‐ and heat‐treated CFSs inhibited >83% of the test strains. Bacteriocin‐like inhibitory substance production of GLB strains can render them valuable probiotics in the control of H. pylori infection.