Covalent Lectin Inhibition and Application in Bacterial Biofilm Imaging. Issue 52 (28th November 2017)

• Record Type: Journal Article
• Authors: Wagner, Stefanie; Hauck, Dirk; Hoffmann, Michael; Sommer, Roman; Joachim, Ines; Müller, Rolf; Imberty, Anne; Varrot, Annabelle; Titz, Alexander
• Is Part Of: Angewandte Chemie international edition. Volume 56:Issue 52(2017)
• Page Start: 16559
• Page End: 16564
• Publication Date: 2017-11-28
• Subjects: biofilms -- carbohydrates -- glycomimetics -- lectins -- Pseudomonas aeruginosa; Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201709368
• Languages: English
• ISSNs: 1433-7851

Abstract

Abstract: Biofilm formation by pathogenic bacteria is a hallmark of chronic infections. In many cases, lectins play key roles in establishing biofilms. The pathogen Pseudomonas aeruginosa often exhibiting various drug resistances employs its lectins LecA and LecB as virulence factors and biofilm building blocks. Therefore, inhibition of the function of these proteins is thought to have potential in developing "pathoblockers" preventing biofilm formation and virulence. A covalent lectin inhibitor specific to a carbohydrate binding site is described for the first time. Its application in the LecA‐specific in vitro imaging of biofilms formed by P. aeruginosa is also reported. Abstract : Biofilm formation by pathogenic bacteria is typical of chronic infections, and lectins often play key roles in establishing biofilms. The drug‐resistant pathogen Pseudomonas aeruginosa employs lectins LecA and LecB as virulence factors and biofilm building blocks. A covalent lectin inhibitor specific to a carbohydrate binding site is described and its application in LecA‐specific in vitro imaging of biofilms formed by P. aeruginosa presented.