Comparative analysis of DNA methylation patterns of equine sarcoid and healthy skin samples. (21st February 2017)

• Record Type: Journal Article
• Authors: Semik, E.; Ząbek, T.; Gurgul, A.; Fornal, A.; Szmatoła, T.; Pawlina, K.; Wnuk, M.; Klukowska‐Rötzler, J.; Koch, C.; Mählmann, K.; Bugno‐Poniewierska, M.
• Is Part Of: Veterinary and comparative oncology. Volume 16:Number 1(2018:Mar.)
• Page Start: 37
• Page End: 46
• Publication Date: 2017-02-21
• Subjects: differentially methylated regions -- DMRs -- horse sarcoids -- methylation -- RRBS -- tumorigenesis; Veterinary oncology -- Periodicals; Neoplasms -- veterinary -- Periodicals; 636.0896994005
• DOI: 10.1111/vco.12308
• Languages: English
• ISSNs: 1476-5810

Abstract

Abstract : Objective: In this study, for the first time we report the genome‐wide DNA methylation profile of skin tumour in horses and describe differentially methylated genomic regions (DMRs) with respect to healthy skin. Materials & Methods: The comparative analysis of DNA methylation patterns detected using Reduced Representation Bisulfite Sequencing (RRBS) technique, allowed identification of 136 regions showing differential methylation between sarcoid and normal skin tissue. Results: Most of the identified DMRs were short fragments, less than 1 kb in size, located in the intergenic regions. Among identified DMRs there were also regions located within genes directly or indirectly related with oncogenesis. We additionally validated 9 CpG sites showing hypomethylation and 9 CpG sites that were hypermethylated in lesional sample, confirming the identified changes in the DNA methylation. Conclusion: Knowledge on the changes taking place in the process of DNA methylation may provide a basis for the development of new alternative diagnostic or therapeutic approaches to equine sarcoids.