Dual‐Responsive Carbon Dot for pH/Redox‐Triggered Fluorescence Imaging with Controllable Photothermal Ablation Therapy of Cancer. (14th June 2018)

• Record Type: Journal Article
• Authors: Choi, Cheong A.; Lee, Jung Eun; Mazrad, Zihnil Adha Islamy; Kim, Young Kwang; In, Insik; Jeong, Ji Hoon; Park, Sung Young
• Is Part Of: ChemMedChem. Volume 13:Number 14(2018)
• Page Start: 1459
• Page End: 1468
• Publication Date: 2018-06-14
• Subjects: controlled release -- fluorescence resonance energy transfer -- fluorescent carbon dots -- photothermal therapy -- redox/pH; Pharmaceutical chemistry -- Periodicals; 615.19005
• DOI: 10.1002/cmdc.201800202
• Languages: English
• ISSNs: 1860-7179

Abstract

Abstract: Herein we describe fluorescence resonance energy transfer (FRET) for a pH/redox‐activatable fluorescent carbon dot (FNP) to realize "off–on" switched imaging‐guided controllable photothermal therapy (PTT). The FNP is a carbonized self‐crosslinked polymer that allows IR825 loading (FNP[IR825]) via hydrophobic interactions for cancer therapy. Fluorescence bioimaging was achieved by the internalization of FNP(IR825) into tumor cells, wherein glutathione (GSH) disulfide bonds are reduced, and benzoic imine groups are cleaved under acidic conditions. The release of IR825 from the FNP core in this system may be used to efficiently control PTT‐mediated cancer therapy via its photothermal conversion after near‐infrared (NIR) irradiation. In vitro and in vivo cellular uptake studies revealed efficient uptake of FNP(IR825) by tumor cells to treat the disease site. In this way we demonstrated in mice that our smart nanocarrier can effectively kill tumor cells under exposure to a NIR laser, and that the particles are biocompatible with various organs. This platform responds sensitively to the exogenous environment inside the cancer cells and may selectively induce the release of PTT‐mediated cytotoxicity. Furthermore, this platform may be useful for monitoring the elimination of cancer cells through the fluorescence on/off switch, which can be used for various applications in the field of cancer cell therapy and diagnosis. Abstract : Flip the switch ! A carbonized self‐crosslinked polymer loaded with IR825 (FNP[IR825]) via hydrophobic interactions was synthesized as a pH/redox‐responsive fluorescent carbon dot (FNP) for "off–on" switched imaging‐guided controllable photothermal therapy (PTT). Fluorescence bioimaging was achieved by the internalization of FNP(IR825) into tumor cells, the reduction of glutathione (GSH) disulfide bonds, and cleavage of benzoic imine under acidic conditions.