Unifoliata‐Afila interactions in pea leaf morphogenesis1. Issue 3 (1st March 2013)

• Record Type: Journal Article
• Authors: DeMason, Darleen A.; Chetty, Venkateswari; Barkawi, Lana S.; Liu, Xing; Cohen, Jerry D.
• Is Part Of: American journal of botany. Volume 100:Issue 3(2013)
• Page Start: 478
• Page End: 495
• Publication Date: 2013-03-01
• Subjects: AFILA -- auxin -- compound leaves -- DR5::GUS -- Fabaceae -- leaf development -- Leguminosae -- pea -- Pisum sativum -- UNIFOLIATA; Botany -- Periodicals; Botany; Electronic journals; Periodicals; 580
• DOI: 10.3732/ajb.1200611
• Languages: English
• ISSNs: 0002-9122

Abstract

Abstract : Premise of the study: Processes of leaf morphogenesis provide the basis for the great diversity of leaf form among higher plants. The common garden pea ( Pisum sativum ) offers a developmental model system for understanding how gene and hormone interactions impart a large array of mutant leaf phenotypes. Methods: To understand the role of auxin in AF and UNI gene function and their interaction, we compared the range of leaf phenotypes on afila ( af ) and unifoliata ( uni ) double mutants, examined the effects of these mutations on auxin levels, auxin transport, auxin response via DR5::GUS, and expression of auxin‐regulated genes. Key results: The adult leaves of af uni double mutants have leaflets and tendrils and typically possess two lateral pinna pairs and a terminal leaflet. The af mutants have higher auxin content, stronger auxin response, and higher expression of auxin responsive genes than wildtype . The uni mutant has reduced auxin content and transport, whereas the uni‐tac mutant has higher auxin content and transport and reduced auxin response compared to wildtype . Conclusions: Auxin concentration and response differences characterize the antagonistic relationship between AF and UNI in pea leaf development. The mechanism involves modulation of auxin mediated by one or both genes; UNI is expressed in and promotes high auxin levels, and AF suppresses auxin levels.