A Highly Photostable Near‐Infrared Labeling Agent Based on a Phospha‐rhodamine for Long‐Term and Deep Imaging. Issue 32 (16th July 2018)

• Record Type: Journal Article
• Authors: Grzybowski, Marek; Taki, Masayasu; Senda, Kieko; Sato, Yoshikatsu; Ariyoshi, Tetsuro; Okada, Yasushi; Kawakami, Ryosuke; Imamura, Takeshi; Yamaguchi, Shigehiro
• Is Part Of: Angewandte Chemie international edition. Volume 57:Issue 32(2018)
• Page Start: 10137
• Page End: 10141
• Publication Date: 2018-07-16
• Subjects: labeling agents -- near-infrared -- optical imaging -- phospha-rhodamine -- photoresistance; Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201804731
• Languages: English
• ISSNs: 1433-7851

Abstract

Abstract: Various fluorescence microscopy techniques require bright NIR‐emitting fluorophores with high chemical and photostability. Now, the significant performance improvement of phosphorus‐substituted rhodamine dyes (PORs) upon substitution at the 9‐position with a 2, 6‐dimethoxyphenyl group is reported. The thus obtained dye PREX 710 was used to stain mitochondria in living cells, which allowed long‐term and three‐color imaging in the vis‐NIR range. Moreover, the high fluorescence longevity of PREX 710 allows tracking a dye‐labeled biomolecule by single‐molecule microscopy under physiological conditions. Deep imaging of blood vessels in mice brain has also been achieved using the bright NIR‐emitting PREX 710‐dextran conjugate. Abstract : Photostable NIR dye : PREX 710 is a P=O‐containing rhodamine dye that is substituted with a 2, 6‐dimethoxyphenyl group at the C9 position. The NIR‐emission and high fluorescence longevity of PREX 710 allow multi‐color imaging, cell‐tracking, and long‐term single‐molecule tracking under physiological conditions as well as deep‐tissue imaging in vivo.