Fiber-bundle illumination: realizing high-degree time-multiplexed multifocal multiphoton microscopy with simplicity. Issue 1 (December 2018)

• Record Type: Journal Article
• Authors: Yu, Jiun-Yann; Kim, Sunduck; Shim, Young; Holland, Daniel; Allodi, Marco; Yeh, Chao-Yuan; Blake, Geoffrey; Han, Young-Geun; Guo, Chin-Lin
• Is Part Of: Scientific reports. Volume 8:Issue 1(2018)
• Page Start: 1
• Page End: 7
• Publication Date: 2018-12
• Subjects: Natural history -- Research -- Periodicals; Biology -- Research -- Periodicals; Physical sciences -- Research -- Periodicals; Earth sciences -- Research -- Periodicals; Environmental sciences -- Research -- Periodicals; 502.85
• DOI: 10.1038/s41598-018-33286-1
• Languages: English
• ISSNs: 2045-2322

Abstract

Abstract High-degree time-multiplexed multifocal multiphoton microscopy was expected to provide a facile path to scanningless optical-sectioning and the fast imaging of dynamic three-dimensional biological systems. However, physical constraints on typical time multiplexing devices, arising from diffraction in the free-space propagation of light waves, lead to significant manufacturing difficulties and have prevented the experimental realization of high-degree time multiplexing. To resolve this issue, we have developed a novel method using optical fiber bundles of various lengths to confine the diffraction of propagating light waves and to create a time multiplexing effect. Through this method, we experimentally demonstrate the highest degree of time multiplexing ever achieved in multifocal multiphoton microscopy (~50 times larger than conventional approaches), and hence the potential of using simply-manufactured devices for scanningless optical sectioning of biological systems.