Elevated and Correlated Expressions of miR-24, miR-30d, miR-146a, and SFRP-4 in Human Abdominal Adipose Tissue Play a Role in Adiposity and Insulin Resistance. (12th March 2018)

• Record Type: Journal Article
• Authors: Nunez Lopez, Yury O.; Garufi, Gabriella; Pasarica, Magdalena; Seyhan, Attila A.
• Other Names: Sebastiani Guido Academic Editor.
• Is Part Of: International journal of endocrinology. Volume 2018(2018)
• Publication Date: 2018-03-12
• Subjects: Endocrinology -- Periodicals; Endocrinology; Endocrinology -- Periodicals; Endocrine System Diseases -- Periodicals; Periodicals; 616.4
• DOI: 10.1155/2018/7351902
• Languages: English
• ISSNs: 1687-8337

Abstract

Abstract : Objective . We explored the relationships among microRNAs (miRNAs) and SFRP4, as they relate to adipose tissue functions including lipolysis, glucose and glycerol turnover, and insulin sensitivity. Methods . Abdominal adipose tissue (AbdAT) levels of thirteen microRNAs (miRNAs), SFRP4, and VEGF in lean nondiabetic subjects (n = 7 ), subjects with obesity (n = 5 ), and subjects with obesity and type 2 diabetes (T2DM) (n = 5 ) were measured by qPCR. Insulin sensitivity was measured by the euglycemic-hyperinsulinemic clamp. Osmium fixation and Coulter counting were used for adipocyte sizing. Data were analyzed using generalized linear models that adjusted for age, gender, and ethnicity. Results . AbdAT miR-24, miR-30d, and miR-146a were elevated in subjects with obesity (P < 0.05 ) and T2DM (P < 0.1 ) and positively correlated with measures of percent body fat by DXA (r m i R . 24 = 0.894, r m i R . 14 6 a = 0.883, P < 0.05 ), and AbdAT SFRP4 (r m i R . 30 = 0.93, r m i R . 14 6 a = 0.88, P < 0.05 ). These three miRNAs additionally correlated among themselves (r m i R . 24 ~ m i R . 14 6 a = 0.90, r m i R . 30 ~ m i R . 14 6 a = 0.85, P < 0.01 ). Conclusions . This study suggests a novel association between the elevated levels of miRNAs miR-24, miR-30d, and miR-146a (apparently coregulated) and the level of SFRP4 transcript in AbdAT of subjects with obesity and T2DM. These molecules might be part of a regulatory loop involved in AbdAT remodeling/adiposity and systemic insulin resistance. This trial is registered withNCT00704197 .