DNA methylation patterns of the S100A14, POU2F3 and SFN genes in equine sarcoid tissues. (August 2018)

• Record Type: Journal Article
• Authors: Semik-Gurgul, E.; Ząbek, T.; Fornal, A.; Wnuk, M.; Pawlina-Tyszko, K.; Gurgul, A.; Klukowska-Rötzler, J.; Koch, C.; Mählmann, K.; Bugno-Poniewierska, M.
• Is Part Of: Research in veterinary science. Volume 119(2018)
• Page Start: 302
• Page End: 307
• Publication Date: 2018-08
• Subjects: Equine sarcoids -- DNA methylation -- SFN -- S100A14 -- POU2F3 -- Tumorigenesis; Veterinary medicine -- Periodicals; Veterinary Medicine -- Periodicals; Médecine vétérinaire -- Périodiques; Médecine vétérinaire -- Recherche -- Périodiques; Diergeneeskunde; 636.089
• DOI: 10.1016/j.rvsc.2018.07.006
• Languages: English
• ISSNs: 0034-5288

Abstract

Abstract: Genetic and epigenetic alterations in the equine sarcoid, a locally invasive skin tumour of equids, are still poorly characterized. Numerous studies have provided reliable evidence for the relationship between the development of cancer and the loss of function of a number of tumour suppressor genes. In the present study, we assessed methylation levels in the promoter region of SFN, S100A14 and POU2F3 genes in sarcoid samples to clarify whether DNA methylation may be associated with previously identified changes in the expression level of these genes during the course of tumour progression. Using bisulfite sequencing and clone sequencing, we detected that lesional samples had a significantly higher rate of DNA methylation in the analyzed S100A14A region than the corresponding normal skin tissue. A frequent methylation of the SFN and POU2F3 promoter sequences were observed in both the tumour samples and the control skin tissues. Further studies are needed to evaluate the role of aberrant methylation in sarcoid progression and to understand the mechanisms involved in reduced expression of SFN, S100A14 and POU2F3 genes in the lesional tissues. Highlights: The DNA methylation changes could be used as the epigenetic cancer markers. The methylation levels of three genes with low expression were evaluated in equine sarcoid samples. The results revealed changes of the DNA methylation level in the analyzed genes. The methylation of the S100A14 gene may be related to the loss of its expression.