Is Total Serum Nitrite and Nitrate (NOx) Level in Dengue Patients a Potential Prognostic Marker of Dengue Hemorrhagic Fever?. (5th July 2018)

• Record Type: Journal Article
• Authors: Mapalagamage, Maheshi; Handunnetti, Shiroma; Premawansa, Gayani; Thillainathan, Sharmila; Fernando, Tharanga; Kanapathippillai, Karunayokiny; Wickremasinghe, Rajitha; De Silva, Aruna Dharshan; Premawansa, Sunil
• Other Names: Hawkes Michael Academic Editor.
• Is Part Of: Disease markers. Volume 2018(2018)
• Publication Date: 2018-07-05
• Subjects: Diagnosis -- Periodicals; Biochemical markers -- Periodicals; Pathology -- Periodicals; 616
• DOI: 10.1155/2018/5328681
• Languages: English
• ISSNs: 0278-0240

Abstract

Abstract : Potential use of total nitrite plus nitrate (NOx) and nitrite (NO2 − ) separately as surrogate markers for serum nitric oxide in severe dengue and their longitudinal changes along with the progression of infection was studied. Deproteinized sera from confirmed dengue fever (DF, n = 145 ) and dengue hemorrhagic fever (DHF, n = 74 ) patients on admission—A, critical—C, discharge—D, and convalescence—CON stages and from age-gender matched healthy individuals (HC, n = 77 ) were taken to assess NO2 − and NOx levels using Griess and modified Griess assays. Serum NOx in DHFA was significantly lower compared to DFA (p < 0.001 ). HC had the lowest NOx and NO2 − compared to all patient categories (p < 0.001 ) except NO2 − in DF-CON and DHF-CON and NOx in DHF-CON. Serum NOx and NO2 − in DHF patients admitted on fever day 3 (DHFA-3) was significantly lower compared to DFA-3 (p < 0.05 ). Cut-off values of 4.46 μ M for NOx (91.3% sensitivity and 80.1% specificity) and 1.25 μ M for NO2 − (75.0% sensitivity and 73.3% specificity) were obtained for day 3 of fever. Serum NOx may be used as potential prognostic marker of DHF in patients presenting with DF in the early stage (on day 3 of fever) of the disease.